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Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage

We aimed to determine the characteristics that distinguish glycosaminoglycans (GAGs) from osteoarthritis (OA) and normal cartilage and from men and women. Cartilage samples from 30 patients subjected to total joint arthroplasty secondary to OA or fracture (control) were evaluated, and the GAG conten...

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Autores principales: Nunes, Rodolfo de Melo, Girão, Virgínia Claudia Carneiro, Cunha, Pablyana Leila Rodrigues, Feitosa, Judith Pessoa Andrade, Pinto, Ana Carolina Matias Dinelly, Rocha, Francisco Airton Castro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116584/
https://www.ncbi.nlm.nih.gov/pubmed/33996844
http://dx.doi.org/10.3389/fmed.2021.612370
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author Nunes, Rodolfo de Melo
Girão, Virgínia Claudia Carneiro
Cunha, Pablyana Leila Rodrigues
Feitosa, Judith Pessoa Andrade
Pinto, Ana Carolina Matias Dinelly
Rocha, Francisco Airton Castro
author_facet Nunes, Rodolfo de Melo
Girão, Virgínia Claudia Carneiro
Cunha, Pablyana Leila Rodrigues
Feitosa, Judith Pessoa Andrade
Pinto, Ana Carolina Matias Dinelly
Rocha, Francisco Airton Castro
author_sort Nunes, Rodolfo de Melo
collection PubMed
description We aimed to determine the characteristics that distinguish glycosaminoglycans (GAGs) from osteoarthritis (OA) and normal cartilage and from men and women. Cartilage samples from 30 patients subjected to total joint arthroplasty secondary to OA or fracture (control) were evaluated, and the GAG content (μg/mg dry cartilage) after proteolysis was determined by densitometry, using agarose-gel electrophoresis. Relative percentages of carbon (C), nitrogen (N), and sulfur (S) in GAGs were determined by elemental microanalysis, as well as the zeta potential. Seventeen samples (56.6%) were from patients >70 years old, with 20 (66.6%) from women, and most [20 (66.6%)] were from the hip. The GAG content was similar regardless of patients being >/≤ 70 years old with 96.5 ± 63.5 and 78.5 ± 38.5 μg/mg (P = 0.1917), respectively. GAG content was higher in women as compared to men, with 89.5 ± 34.3 and 51.8 ± 13.3 μg/mg, respectively (P = 0.0022), as well as in OA than fracture samples, with 98.4 ± 63.5 and 63.6 ± 19.6 μg/mg, respectively (P = 0.0355). The GAG extracted from the cartilage of patients >70 years old had increase in N, and there were no gender differences regarding GAG elemental analysis. GAG from OA had a highly significant (P = 0.0005) decrease in S% (1.79% ± 0.25%), as compared to fracture samples (2.3% ± 0.19%), with an associated and significant (P = 0.0001) reduction of the zeta potential in the OA group. This is the first report of a reduced S content in GAG from OA patients, which is associated with a reduced zeta potential.
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spelling pubmed-81165842021-05-14 Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage Nunes, Rodolfo de Melo Girão, Virgínia Claudia Carneiro Cunha, Pablyana Leila Rodrigues Feitosa, Judith Pessoa Andrade Pinto, Ana Carolina Matias Dinelly Rocha, Francisco Airton Castro Front Med (Lausanne) Medicine We aimed to determine the characteristics that distinguish glycosaminoglycans (GAGs) from osteoarthritis (OA) and normal cartilage and from men and women. Cartilage samples from 30 patients subjected to total joint arthroplasty secondary to OA or fracture (control) were evaluated, and the GAG content (μg/mg dry cartilage) after proteolysis was determined by densitometry, using agarose-gel electrophoresis. Relative percentages of carbon (C), nitrogen (N), and sulfur (S) in GAGs were determined by elemental microanalysis, as well as the zeta potential. Seventeen samples (56.6%) were from patients >70 years old, with 20 (66.6%) from women, and most [20 (66.6%)] were from the hip. The GAG content was similar regardless of patients being >/≤ 70 years old with 96.5 ± 63.5 and 78.5 ± 38.5 μg/mg (P = 0.1917), respectively. GAG content was higher in women as compared to men, with 89.5 ± 34.3 and 51.8 ± 13.3 μg/mg, respectively (P = 0.0022), as well as in OA than fracture samples, with 98.4 ± 63.5 and 63.6 ± 19.6 μg/mg, respectively (P = 0.0355). The GAG extracted from the cartilage of patients >70 years old had increase in N, and there were no gender differences regarding GAG elemental analysis. GAG from OA had a highly significant (P = 0.0005) decrease in S% (1.79% ± 0.25%), as compared to fracture samples (2.3% ± 0.19%), with an associated and significant (P = 0.0001) reduction of the zeta potential in the OA group. This is the first report of a reduced S content in GAG from OA patients, which is associated with a reduced zeta potential. Frontiers Media S.A. 2021-04-29 /pmc/articles/PMC8116584/ /pubmed/33996844 http://dx.doi.org/10.3389/fmed.2021.612370 Text en Copyright © 2021 Nunes, Girão, Cunha, Feitosa, Pinto and Rocha. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Nunes, Rodolfo de Melo
Girão, Virgínia Claudia Carneiro
Cunha, Pablyana Leila Rodrigues
Feitosa, Judith Pessoa Andrade
Pinto, Ana Carolina Matias Dinelly
Rocha, Francisco Airton Castro
Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage
title Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage
title_full Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage
title_fullStr Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage
title_full_unstemmed Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage
title_short Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage
title_sort decreased sulfate content and zeta potential distinguish glycosaminoglycans of the extracellular matrix of osteoarthritis cartilage
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116584/
https://www.ncbi.nlm.nih.gov/pubmed/33996844
http://dx.doi.org/10.3389/fmed.2021.612370
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