Cargando…

Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas

Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Ci...

Descripción completa

Detalles Bibliográficos
Autores principales: Chabi-Jesus, Camila, Ramos-González, Pedro L., Postclam-Barro, Matheus, Fontenele, Rafaela Salgado, Harakava, Ricardo, Bassanezi, Renato B., Moreira, Alecio S., Kitajima, Elliot W., Varsani, Arvind, Freitas-Astúa, Juliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116597/
https://www.ncbi.nlm.nih.gov/pubmed/33995302
http://dx.doi.org/10.3389/fmicb.2021.641252
_version_ 1783691428408328192
author Chabi-Jesus, Camila
Ramos-González, Pedro L.
Postclam-Barro, Matheus
Fontenele, Rafaela Salgado
Harakava, Ricardo
Bassanezi, Renato B.
Moreira, Alecio S.
Kitajima, Elliot W.
Varsani, Arvind
Freitas-Astúa, Juliana
author_facet Chabi-Jesus, Camila
Ramos-González, Pedro L.
Postclam-Barro, Matheus
Fontenele, Rafaela Salgado
Harakava, Ricardo
Bassanezi, Renato B.
Moreira, Alecio S.
Kitajima, Elliot W.
Varsani, Arvind
Freitas-Astúa, Juliana
author_sort Chabi-Jesus, Camila
collection PubMed
description Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ∼1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region.
format Online
Article
Text
id pubmed-8116597
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81165972021-05-14 Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas Chabi-Jesus, Camila Ramos-González, Pedro L. Postclam-Barro, Matheus Fontenele, Rafaela Salgado Harakava, Ricardo Bassanezi, Renato B. Moreira, Alecio S. Kitajima, Elliot W. Varsani, Arvind Freitas-Astúa, Juliana Front Microbiol Microbiology Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ∼1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region. Frontiers Media S.A. 2021-04-29 /pmc/articles/PMC8116597/ /pubmed/33995302 http://dx.doi.org/10.3389/fmicb.2021.641252 Text en Copyright © 2021 Chabi-Jesus, Ramos-González, Postclam-Barro, Fontenele, Harakava, Bassanezi, Moreira, Kitajima, Varsani and Freitas-Astúa. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Chabi-Jesus, Camila
Ramos-González, Pedro L.
Postclam-Barro, Matheus
Fontenele, Rafaela Salgado
Harakava, Ricardo
Bassanezi, Renato B.
Moreira, Alecio S.
Kitajima, Elliot W.
Varsani, Arvind
Freitas-Astúa, Juliana
Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas
title Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas
title_full Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas
title_fullStr Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas
title_full_unstemmed Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas
title_short Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas
title_sort molecular epidemiology of citrus leprosis virus c: a new viral lineage and phylodynamic of the main viral subpopulations in the americas
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116597/
https://www.ncbi.nlm.nih.gov/pubmed/33995302
http://dx.doi.org/10.3389/fmicb.2021.641252
work_keys_str_mv AT chabijesuscamila molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT ramosgonzalezpedrol molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT postclambarromatheus molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT fontenelerafaelasalgado molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT harakavaricardo molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT bassanezirenatob molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT moreiraalecios molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT kitajimaelliotw molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT varsaniarvind molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas
AT freitasastuajuliana molecularepidemiologyofcitrusleprosisviruscanewvirallineageandphylodynamicofthemainviralsubpopulationsintheamericas