Generation and evaluation of IgY-scFv based mimetics against canine parvovirus

Antibody mimetics may be used for various biomedical applications, especially those for which conventional antibodies are ineffective. In this study, we developed a smaller molecular chicken IgY mimetic peptide (IgY-peptide) based on the complementarity-determining regions (CDRs) of the anti-canine...

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Autores principales: Ge, Shikun, Zhang, Xingxing, Zhong, Fagang, Liu, Xiang, Zhang, Xiaoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116823/
https://www.ncbi.nlm.nih.gov/pubmed/33985573
http://dx.doi.org/10.1186/s13567-021-00943-9
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author Ge, Shikun
Zhang, Xingxing
Zhong, Fagang
Liu, Xiang
Zhang, Xiaoying
author_facet Ge, Shikun
Zhang, Xingxing
Zhong, Fagang
Liu, Xiang
Zhang, Xiaoying
author_sort Ge, Shikun
collection PubMed
description Antibody mimetics may be used for various biomedical applications, especially those for which conventional antibodies are ineffective. In this study, we developed a smaller molecular chicken IgY mimetic peptide (IgY-peptide) based on the complementarity-determining regions (CDRs) of the anti-canine parvovirus (CPV) IgY-scFv prepared previously. The mimetic peptide showed no cross-reactivity with canine distemper virus (CDV) and canine coronavirus (CCV) and showed excellent protective properties for Crandell-Rees Feline Kidney (CRFK) cells against CPV. This study is the first attempt to develop a mimetic IgY-peptide and demonstrates the ease and feasibility in generating such a novel antibody-like functional molecule for biomedical purposes.
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spelling pubmed-81168232021-05-13 Generation and evaluation of IgY-scFv based mimetics against canine parvovirus Ge, Shikun Zhang, Xingxing Zhong, Fagang Liu, Xiang Zhang, Xiaoying Vet Res Short Report Antibody mimetics may be used for various biomedical applications, especially those for which conventional antibodies are ineffective. In this study, we developed a smaller molecular chicken IgY mimetic peptide (IgY-peptide) based on the complementarity-determining regions (CDRs) of the anti-canine parvovirus (CPV) IgY-scFv prepared previously. The mimetic peptide showed no cross-reactivity with canine distemper virus (CDV) and canine coronavirus (CCV) and showed excellent protective properties for Crandell-Rees Feline Kidney (CRFK) cells against CPV. This study is the first attempt to develop a mimetic IgY-peptide and demonstrates the ease and feasibility in generating such a novel antibody-like functional molecule for biomedical purposes. BioMed Central 2021-05-13 2021 /pmc/articles/PMC8116823/ /pubmed/33985573 http://dx.doi.org/10.1186/s13567-021-00943-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Ge, Shikun
Zhang, Xingxing
Zhong, Fagang
Liu, Xiang
Zhang, Xiaoying
Generation and evaluation of IgY-scFv based mimetics against canine parvovirus
title Generation and evaluation of IgY-scFv based mimetics against canine parvovirus
title_full Generation and evaluation of IgY-scFv based mimetics against canine parvovirus
title_fullStr Generation and evaluation of IgY-scFv based mimetics against canine parvovirus
title_full_unstemmed Generation and evaluation of IgY-scFv based mimetics against canine parvovirus
title_short Generation and evaluation of IgY-scFv based mimetics against canine parvovirus
title_sort generation and evaluation of igy-scfv based mimetics against canine parvovirus
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116823/
https://www.ncbi.nlm.nih.gov/pubmed/33985573
http://dx.doi.org/10.1186/s13567-021-00943-9
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