Optimizing expression of a single copy transgene in C. elegans

The utility of single copy transgenic insertions in C. elegans is often limited by low expression. We examined the effects of modifying the trans-splicing signal, the Kozak ribosome binding site, the N-terminal amino acid of the reporter and the 3′ UTR sequences on the expression level of a mec-4 promoter GFP transgene. The trans-splicing signal and the 3′ UTR had most dramatic effects on expression while modifying the Kozak signal or the N-terminal amino acid had less influence on expression.