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Optimizing expression of a single copy transgene in C. elegans
The utility of single copy transgenic insertions in C. elegans is often limited by low expression. We examined the effects of modifying the trans-splicing signal, the Kozak ribosome binding site, the N-terminal amino acid of the reporter and the 3′ UTR sequences on the expression level of a mec-4 pr...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Caltech Library
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116934/ https://www.ncbi.nlm.nih.gov/pubmed/33997659 http://dx.doi.org/10.17912/micropub.biology.000394 |