Cargando…
TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model pl...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117018/ https://www.ncbi.nlm.nih.gov/pubmed/33995462 http://dx.doi.org/10.3389/fpls.2021.666229 |
_version_ | 1783691521504051200 |
---|---|
author | Takáč, Tomáš Křenek, Pavel Komis, George Vadovič, Pavol Ovečka, Miroslav Ohnoutková, Ludmila Pechan, Tibor Kašpárek, Petr Tichá, Tereza Basheer, Jasim Arick, Mark Šamaj, Jozef |
author_facet | Takáč, Tomáš Křenek, Pavel Komis, George Vadovič, Pavol Ovečka, Miroslav Ohnoutková, Ludmila Pechan, Tibor Kašpárek, Petr Tichá, Tereza Basheer, Jasim Arick, Mark Šamaj, Jozef |
author_sort | Takáč, Tomáš |
collection | PubMed |
description | Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22. |
format | Online Article Text |
id | pubmed-8117018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81170182021-05-14 TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley Takáč, Tomáš Křenek, Pavel Komis, George Vadovič, Pavol Ovečka, Miroslav Ohnoutková, Ludmila Pechan, Tibor Kašpárek, Petr Tichá, Tereza Basheer, Jasim Arick, Mark Šamaj, Jozef Front Plant Sci Plant Science Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22. Frontiers Media S.A. 2021-04-29 /pmc/articles/PMC8117018/ /pubmed/33995462 http://dx.doi.org/10.3389/fpls.2021.666229 Text en Copyright © 2021 Takáč, Křenek, Komis, Vadovič, Ovečka, Ohnoutková, Pechan, Kašpárek, Tichá, Basheer, Arick and Šamaj. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Takáč, Tomáš Křenek, Pavel Komis, George Vadovič, Pavol Ovečka, Miroslav Ohnoutková, Ludmila Pechan, Tibor Kašpárek, Petr Tichá, Tereza Basheer, Jasim Arick, Mark Šamaj, Jozef TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley |
title | TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley |
title_full | TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley |
title_fullStr | TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley |
title_full_unstemmed | TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley |
title_short | TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley |
title_sort | talen-based hvmpk3 knock-out attenuates proteome and root hair phenotypic responses to flg22 in barley |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117018/ https://www.ncbi.nlm.nih.gov/pubmed/33995462 http://dx.doi.org/10.3389/fpls.2021.666229 |
work_keys_str_mv | AT takactomas talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT krenekpavel talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT komisgeorge talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT vadovicpavol talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT oveckamiroslav talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT ohnoutkovaludmila talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT pechantibor talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT kasparekpetr talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT tichatereza talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT basheerjasim talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT arickmark talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley AT samajjozef talenbasedhvmpk3knockoutattenuatesproteomeandroothairphenotypicresponsestoflg22inbarley |