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TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley

Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model pl...

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Autores principales: Takáč, Tomáš, Křenek, Pavel, Komis, George, Vadovič, Pavol, Ovečka, Miroslav, Ohnoutková, Ludmila, Pechan, Tibor, Kašpárek, Petr, Tichá, Tereza, Basheer, Jasim, Arick, Mark, Šamaj, Jozef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117018/
https://www.ncbi.nlm.nih.gov/pubmed/33995462
http://dx.doi.org/10.3389/fpls.2021.666229
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author Takáč, Tomáš
Křenek, Pavel
Komis, George
Vadovič, Pavol
Ovečka, Miroslav
Ohnoutková, Ludmila
Pechan, Tibor
Kašpárek, Petr
Tichá, Tereza
Basheer, Jasim
Arick, Mark
Šamaj, Jozef
author_facet Takáč, Tomáš
Křenek, Pavel
Komis, George
Vadovič, Pavol
Ovečka, Miroslav
Ohnoutková, Ludmila
Pechan, Tibor
Kašpárek, Petr
Tichá, Tereza
Basheer, Jasim
Arick, Mark
Šamaj, Jozef
author_sort Takáč, Tomáš
collection PubMed
description Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22.
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spelling pubmed-81170182021-05-14 TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley Takáč, Tomáš Křenek, Pavel Komis, George Vadovič, Pavol Ovečka, Miroslav Ohnoutková, Ludmila Pechan, Tibor Kašpárek, Petr Tichá, Tereza Basheer, Jasim Arick, Mark Šamaj, Jozef Front Plant Sci Plant Science Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22. Frontiers Media S.A. 2021-04-29 /pmc/articles/PMC8117018/ /pubmed/33995462 http://dx.doi.org/10.3389/fpls.2021.666229 Text en Copyright © 2021 Takáč, Křenek, Komis, Vadovič, Ovečka, Ohnoutková, Pechan, Kašpárek, Tichá, Basheer, Arick and Šamaj. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Takáč, Tomáš
Křenek, Pavel
Komis, George
Vadovič, Pavol
Ovečka, Miroslav
Ohnoutková, Ludmila
Pechan, Tibor
Kašpárek, Petr
Tichá, Tereza
Basheer, Jasim
Arick, Mark
Šamaj, Jozef
TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
title TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
title_full TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
title_fullStr TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
title_full_unstemmed TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
title_short TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
title_sort talen-based hvmpk3 knock-out attenuates proteome and root hair phenotypic responses to flg22 in barley
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117018/
https://www.ncbi.nlm.nih.gov/pubmed/33995462
http://dx.doi.org/10.3389/fpls.2021.666229
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