Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran

BACKGROUND: Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods...

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Autores principales: Heshmati Afshar, Fariba, Zadehkamand, Masumeh, Rezaei, Zahra, Delazar, Abbas, Tarhriz, Vahideh, Asgharian, Parina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
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Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117297/
https://www.ncbi.nlm.nih.gov/pubmed/33980281
http://dx.doi.org/10.1186/s13065-021-00759-w
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author Heshmati Afshar, Fariba
Zadehkamand, Masumeh
Rezaei, Zahra
Delazar, Abbas
Tarhriz, Vahideh
Asgharian, Parina
author_facet Heshmati Afshar, Fariba
Zadehkamand, Masumeh
Rezaei, Zahra
Delazar, Abbas
Tarhriz, Vahideh
Asgharian, Parina
author_sort Heshmati Afshar, Fariba
collection PubMed
description BACKGROUND: Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by (1)H and (13)C NMR. RESULTS: The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides. CONCLUSION: Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics.
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spelling pubmed-81172972021-05-13 Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran Heshmati Afshar, Fariba Zadehkamand, Masumeh Rezaei, Zahra Delazar, Abbas Tarhriz, Vahideh Asgharian, Parina BMC Chem Research Article BACKGROUND: Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by (1)H and (13)C NMR. RESULTS: The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides. CONCLUSION: Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics. Springer International Publishing 2021-05-12 /pmc/articles/PMC8117297/ /pubmed/33980281 http://dx.doi.org/10.1186/s13065-021-00759-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Heshmati Afshar, Fariba
Zadehkamand, Masumeh
Rezaei, Zahra
Delazar, Abbas
Tarhriz, Vahideh
Asgharian, Parina
Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran
title Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran
title_full Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran
title_fullStr Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran
title_full_unstemmed Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran
title_short Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran
title_sort chemical compositions, antimicrobial effects, and cytotoxicity of asia minor wormwood (artemisia splendens willd.) growing in iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117297/
https://www.ncbi.nlm.nih.gov/pubmed/33980281
http://dx.doi.org/10.1186/s13065-021-00759-w
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