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Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR

Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly detected. Serovars of greatest clinical relevance were systemic isolate...

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Autores principales: Macedo, Nubia, Gottschalk, Marcelo, Strutzberg-Minder, Katrin, Van, Chao Nguyen, Zhang, Lijun, Zou, Geng, Zhou, Rui, Marostica, Thaire, Clavijo, Maria Jose, Tucker, Alexander, Aragon, Virginia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117636/
https://www.ncbi.nlm.nih.gov/pubmed/33980312
http://dx.doi.org/10.1186/s13567-021-00935-9
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author Macedo, Nubia
Gottschalk, Marcelo
Strutzberg-Minder, Katrin
Van, Chao Nguyen
Zhang, Lijun
Zou, Geng
Zhou, Rui
Marostica, Thaire
Clavijo, Maria Jose
Tucker, Alexander
Aragon, Virginia
author_facet Macedo, Nubia
Gottschalk, Marcelo
Strutzberg-Minder, Katrin
Van, Chao Nguyen
Zhang, Lijun
Zou, Geng
Zhou, Rui
Marostica, Thaire
Clavijo, Maria Jose
Tucker, Alexander
Aragon, Virginia
author_sort Macedo, Nubia
collection PubMed
description Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly detected. Serovars of greatest clinical relevance were systemic isolates that had a higher probability of being serovar 5/12, 13, or 7. In comparison, pulmonary isolates had a higher likelihood of being serovars 2, 4, 7, or 14. Serovars 5/12 and 13 have previously been considered disease-associated, but this study agrees with other recent studies showing that serovar 7 is indeed associated with systemic G. parasuis disease. Serovar 4 strains illustrated how isolates can have varying degrees of virulence and be obtained from pulmonary, systemic, or nasal sites. Serovars 8, 9, 15, and 10 were predominantly obtained from nasal samples, which indicates a limited clinical significance of these serovars. Additionally, most internal G. parasuis isolates were classified as virulent by LS-PCR and were disease-associated isolates, including serovars 1, 2, 4, 5/12, 7, 13, and 14. Isolates from the nasal cavity, including serovars 6, 9, 10, 11, and 15, were classified as non-virulent by LS-PCR. In conclusion, the distribution of G. parasuis serovars remains constant, with few serovars representing most of the strains isolated from affected pigs. Moreover, it was confirmed that the LS-PCR can be used for G. parasuis virulence prediction of field strains worldwide. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-021-00935-9.
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spelling pubmed-81176362021-05-17 Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR Macedo, Nubia Gottschalk, Marcelo Strutzberg-Minder, Katrin Van, Chao Nguyen Zhang, Lijun Zou, Geng Zhou, Rui Marostica, Thaire Clavijo, Maria Jose Tucker, Alexander Aragon, Virginia Vet Res Research Article Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly detected. Serovars of greatest clinical relevance were systemic isolates that had a higher probability of being serovar 5/12, 13, or 7. In comparison, pulmonary isolates had a higher likelihood of being serovars 2, 4, 7, or 14. Serovars 5/12 and 13 have previously been considered disease-associated, but this study agrees with other recent studies showing that serovar 7 is indeed associated with systemic G. parasuis disease. Serovar 4 strains illustrated how isolates can have varying degrees of virulence and be obtained from pulmonary, systemic, or nasal sites. Serovars 8, 9, 15, and 10 were predominantly obtained from nasal samples, which indicates a limited clinical significance of these serovars. Additionally, most internal G. parasuis isolates were classified as virulent by LS-PCR and were disease-associated isolates, including serovars 1, 2, 4, 5/12, 7, 13, and 14. Isolates from the nasal cavity, including serovars 6, 9, 10, 11, and 15, were classified as non-virulent by LS-PCR. In conclusion, the distribution of G. parasuis serovars remains constant, with few serovars representing most of the strains isolated from affected pigs. Moreover, it was confirmed that the LS-PCR can be used for G. parasuis virulence prediction of field strains worldwide. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-021-00935-9. BioMed Central 2021-05-12 2021 /pmc/articles/PMC8117636/ /pubmed/33980312 http://dx.doi.org/10.1186/s13567-021-00935-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Macedo, Nubia
Gottschalk, Marcelo
Strutzberg-Minder, Katrin
Van, Chao Nguyen
Zhang, Lijun
Zou, Geng
Zhou, Rui
Marostica, Thaire
Clavijo, Maria Jose
Tucker, Alexander
Aragon, Virginia
Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
title Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
title_full Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
title_fullStr Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
title_full_unstemmed Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
title_short Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
title_sort molecular characterization of glaesserella parasuis strains isolated from north america, europe and asia by serotyping pcr and ls-pcr
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117636/
https://www.ncbi.nlm.nih.gov/pubmed/33980312
http://dx.doi.org/10.1186/s13567-021-00935-9
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