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Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis

Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after l...

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Autores principales: Kim, Hyunseong, Hong, Jin Young, Jeon, Wan-Jin, Lee, Junseon, Ha, In-Hyuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118556/
https://www.ncbi.nlm.nih.gov/pubmed/33984013
http://dx.doi.org/10.1371/journal.pone.0251464
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author Kim, Hyunseong
Hong, Jin Young
Jeon, Wan-Jin
Lee, Junseon
Ha, In-Hyuk
author_facet Kim, Hyunseong
Hong, Jin Young
Jeon, Wan-Jin
Lee, Junseon
Ha, In-Hyuk
author_sort Kim, Hyunseong
collection PubMed
description Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after laminectomy. However, the effects of differences between these implant materials have not been studied. We assessed the degree of damage and locomotor function of an LSS model in Sprague-Dawley rats using silicone blocks of varying hardness (70, 80, and 90 kPa) implanted at the L4 level. In sham rats, the spinal cord remained intact; in LSS rats, the spinal cord was increasingly compressed by the mechanical pressure of the silicone blocks as hardness increased. Inflammatory cells were not evident in sham rats, but numerous inflammatory cells were observed around the implanted silicone block in LSS rats. CD68+ cell quantification revealed increases in the inflammatory response in a hardness-dependent manner in LSS rats. Compared with those in sham rats, proinflammatory cytokine levels were significantly elevated in a hardness-dependent manner, and locomotor function was significantly decreased, in LSS rats. Overall, this study showed that hardness could be used as an index to control the severity of nerve injury induced by silicone implants.
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spelling pubmed-81185562021-05-24 Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis Kim, Hyunseong Hong, Jin Young Jeon, Wan-Jin Lee, Junseon Ha, In-Hyuk PLoS One Research Article Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after laminectomy. However, the effects of differences between these implant materials have not been studied. We assessed the degree of damage and locomotor function of an LSS model in Sprague-Dawley rats using silicone blocks of varying hardness (70, 80, and 90 kPa) implanted at the L4 level. In sham rats, the spinal cord remained intact; in LSS rats, the spinal cord was increasingly compressed by the mechanical pressure of the silicone blocks as hardness increased. Inflammatory cells were not evident in sham rats, but numerous inflammatory cells were observed around the implanted silicone block in LSS rats. CD68+ cell quantification revealed increases in the inflammatory response in a hardness-dependent manner in LSS rats. Compared with those in sham rats, proinflammatory cytokine levels were significantly elevated in a hardness-dependent manner, and locomotor function was significantly decreased, in LSS rats. Overall, this study showed that hardness could be used as an index to control the severity of nerve injury induced by silicone implants. Public Library of Science 2021-05-13 /pmc/articles/PMC8118556/ /pubmed/33984013 http://dx.doi.org/10.1371/journal.pone.0251464 Text en © 2021 Kim et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kim, Hyunseong
Hong, Jin Young
Jeon, Wan-Jin
Lee, Junseon
Ha, In-Hyuk
Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis
title Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis
title_full Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis
title_fullStr Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis
title_full_unstemmed Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis
title_short Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis
title_sort evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118556/
https://www.ncbi.nlm.nih.gov/pubmed/33984013
http://dx.doi.org/10.1371/journal.pone.0251464
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