Cargando…
Microbiological Testing for Coronavirus Disease 2019
Microbiological diagnosis of coronavirus disease 2019 (COVID-19) is mainly performed through nucleic acid amplification test (NAAT) and antigen test. Although NAAT is the standard diagnostic test, its use is limited by insufficient laboratory resources and long turnaround time. Point-of-care NAAT te...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japan Medical Association
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119126/ https://www.ncbi.nlm.nih.gov/pubmed/33997438 http://dx.doi.org/10.31662/jmaj.2021-0012 |
_version_ | 1783691817009545216 |
---|---|
author | Yamamoto, Kei Ohmagari, Norio |
author_facet | Yamamoto, Kei Ohmagari, Norio |
author_sort | Yamamoto, Kei |
collection | PubMed |
description | Microbiological diagnosis of coronavirus disease 2019 (COVID-19) is mainly performed through nucleic acid amplification test (NAAT) and antigen test. Although NAAT is the standard diagnostic test, its use is limited by insufficient laboratory resources and long turnaround time. Point-of-care NAAT tests have been introduced to address these shortcomings, but their varied sensitivity and resource constraints remain a concern. Antigen tests require fewer resources but have low sensitivity. Nevertheless, low-sensitivity tests may be useful depending on the situation. In contrast, in some clinical phases of COVID-19, high-sensitivity tests may provide false-negative results. Therefore, the right testing strategy is needed for an accurate diagnosis. In this review, the characteristics and clinical applications of microbiological tests available in Japan (NAAT, antigen test, and antibody test) are discussed. The clinical diagnosis of COVID-19 is slightly complicated, and cases in which the infection spreads from asymptomatic infected individuals are many; hence, laboratory diagnosis is essential to prevent further transmission. |
format | Online Article Text |
id | pubmed-8119126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Japan Medical Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-81191262021-05-14 Microbiological Testing for Coronavirus Disease 2019 Yamamoto, Kei Ohmagari, Norio JMA J Review Article: COVID-19 Microbiological diagnosis of coronavirus disease 2019 (COVID-19) is mainly performed through nucleic acid amplification test (NAAT) and antigen test. Although NAAT is the standard diagnostic test, its use is limited by insufficient laboratory resources and long turnaround time. Point-of-care NAAT tests have been introduced to address these shortcomings, but their varied sensitivity and resource constraints remain a concern. Antigen tests require fewer resources but have low sensitivity. Nevertheless, low-sensitivity tests may be useful depending on the situation. In contrast, in some clinical phases of COVID-19, high-sensitivity tests may provide false-negative results. Therefore, the right testing strategy is needed for an accurate diagnosis. In this review, the characteristics and clinical applications of microbiological tests available in Japan (NAAT, antigen test, and antibody test) are discussed. The clinical diagnosis of COVID-19 is slightly complicated, and cases in which the infection spreads from asymptomatic infected individuals are many; hence, laboratory diagnosis is essential to prevent further transmission. Japan Medical Association 2021-04-02 2021-04-15 /pmc/articles/PMC8119126/ /pubmed/33997438 http://dx.doi.org/10.31662/jmaj.2021-0012 Text en Copyright © Japan Medical Association https://creativecommons.org/licenses/by/4.0/JMA Journal is an Open Access journal distributed under the Creative Commons Attribution 4.0 International License. To view the details of this license, please visit (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Review Article: COVID-19 Yamamoto, Kei Ohmagari, Norio Microbiological Testing for Coronavirus Disease 2019 |
title | Microbiological Testing for Coronavirus Disease 2019 |
title_full | Microbiological Testing for Coronavirus Disease 2019 |
title_fullStr | Microbiological Testing for Coronavirus Disease 2019 |
title_full_unstemmed | Microbiological Testing for Coronavirus Disease 2019 |
title_short | Microbiological Testing for Coronavirus Disease 2019 |
title_sort | microbiological testing for coronavirus disease 2019 |
topic | Review Article: COVID-19 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119126/ https://www.ncbi.nlm.nih.gov/pubmed/33997438 http://dx.doi.org/10.31662/jmaj.2021-0012 |
work_keys_str_mv | AT yamamotokei microbiologicaltestingforcoronavirusdisease2019 AT ohmagarinorio microbiologicaltestingforcoronavirusdisease2019 |