Cargando…
Vaccine delivery alerts innate immune systems for more immunogenic vaccination
Vaccine delivery technologies are mainly designed to minimally invasively deliver vaccines to target tissues with little or no adjuvant effects. This study presents a prototype laser-based powder delivery (LPD) with inherent adjuvant effects for more immunogenic vaccination without incorporation of...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Clinical Investigation
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119203/ https://www.ncbi.nlm.nih.gov/pubmed/33690222 http://dx.doi.org/10.1172/jci.insight.144627 |
_version_ | 1783691823753986048 |
---|---|
author | Li, Zhuofan Cao, Yan Li, Yibo Zhao, Yiwen Chen, Xinyuan |
author_facet | Li, Zhuofan Cao, Yan Li, Yibo Zhao, Yiwen Chen, Xinyuan |
author_sort | Li, Zhuofan |
collection | PubMed |
description | Vaccine delivery technologies are mainly designed to minimally invasively deliver vaccines to target tissues with little or no adjuvant effects. This study presents a prototype laser-based powder delivery (LPD) with inherent adjuvant effects for more immunogenic vaccination without incorporation of external adjuvants. LPD takes advantage of aesthetic ablative fractional laser to generate skin microchannels to support high-efficient vaccine delivery and at the same time creates photothermal stress in microchannel-surrounding tissues to boost vaccination. LPD could significantly enhance pandemic influenza 2009 H1N1 vaccine immunogenicity and protective efficacy as compared with needle-based intradermal delivery in murine models. The ablative fractional laser was found to induce host DNA release, activate NLR family pyrin domain containing 3 inflammasome, and stimulate IL-1β release despite their dispensability for laser adjuvant effects. Instead, the ablative fractional laser activated MyD88 to mediate its adjuvant effects by potentiation of antigen uptake, maturation, and migration of dendritic cells. LPD also induced minimal local or systemic adverse reactions due to the microfractional and sustained vaccine delivery. Our data support the development of self-adjuvanted vaccine delivery technologies by intentional induction of well-controlled tissue stress to alert innate immune systems for more immunogenic vaccination. |
format | Online Article Text |
id | pubmed-8119203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Clinical Investigation |
record_format | MEDLINE/PubMed |
spelling | pubmed-81192032021-05-18 Vaccine delivery alerts innate immune systems for more immunogenic vaccination Li, Zhuofan Cao, Yan Li, Yibo Zhao, Yiwen Chen, Xinyuan JCI Insight Research Article Vaccine delivery technologies are mainly designed to minimally invasively deliver vaccines to target tissues with little or no adjuvant effects. This study presents a prototype laser-based powder delivery (LPD) with inherent adjuvant effects for more immunogenic vaccination without incorporation of external adjuvants. LPD takes advantage of aesthetic ablative fractional laser to generate skin microchannels to support high-efficient vaccine delivery and at the same time creates photothermal stress in microchannel-surrounding tissues to boost vaccination. LPD could significantly enhance pandemic influenza 2009 H1N1 vaccine immunogenicity and protective efficacy as compared with needle-based intradermal delivery in murine models. The ablative fractional laser was found to induce host DNA release, activate NLR family pyrin domain containing 3 inflammasome, and stimulate IL-1β release despite their dispensability for laser adjuvant effects. Instead, the ablative fractional laser activated MyD88 to mediate its adjuvant effects by potentiation of antigen uptake, maturation, and migration of dendritic cells. LPD also induced minimal local or systemic adverse reactions due to the microfractional and sustained vaccine delivery. Our data support the development of self-adjuvanted vaccine delivery technologies by intentional induction of well-controlled tissue stress to alert innate immune systems for more immunogenic vaccination. American Society for Clinical Investigation 2021-04-08 /pmc/articles/PMC8119203/ /pubmed/33690222 http://dx.doi.org/10.1172/jci.insight.144627 Text en © 2021 Li et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Li, Zhuofan Cao, Yan Li, Yibo Zhao, Yiwen Chen, Xinyuan Vaccine delivery alerts innate immune systems for more immunogenic vaccination |
title | Vaccine delivery alerts innate immune systems for more immunogenic vaccination |
title_full | Vaccine delivery alerts innate immune systems for more immunogenic vaccination |
title_fullStr | Vaccine delivery alerts innate immune systems for more immunogenic vaccination |
title_full_unstemmed | Vaccine delivery alerts innate immune systems for more immunogenic vaccination |
title_short | Vaccine delivery alerts innate immune systems for more immunogenic vaccination |
title_sort | vaccine delivery alerts innate immune systems for more immunogenic vaccination |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119203/ https://www.ncbi.nlm.nih.gov/pubmed/33690222 http://dx.doi.org/10.1172/jci.insight.144627 |
work_keys_str_mv | AT lizhuofan vaccinedeliveryalertsinnateimmunesystemsformoreimmunogenicvaccination AT caoyan vaccinedeliveryalertsinnateimmunesystemsformoreimmunogenicvaccination AT liyibo vaccinedeliveryalertsinnateimmunesystemsformoreimmunogenicvaccination AT zhaoyiwen vaccinedeliveryalertsinnateimmunesystemsformoreimmunogenicvaccination AT chenxinyuan vaccinedeliveryalertsinnateimmunesystemsformoreimmunogenicvaccination |