Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1

We show that ivermectin, an FDA-approved anti-parasitic drug, effectively inhibits infection with hepatitis E virus (HEV) genotypes 1 and 3 in a range of cell culture models, including hepatic and extrahepatic cells. Long-term treatment showed no clear evidence of the development of drug resistance....

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Autores principales: Li, Yunlong, Miao, Zhijiang, Li, Pengfei, Zhang, Ruyi, Kainov, Denis E., Ma, Zhongren, de Man, Robert A., Peppelenbosch, Maikel P., Pan, Qiuwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119233/
https://www.ncbi.nlm.nih.gov/pubmed/33987753
http://dx.doi.org/10.1007/s00705-021-05096-w
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author Li, Yunlong
Miao, Zhijiang
Li, Pengfei
Zhang, Ruyi
Kainov, Denis E.
Ma, Zhongren
de Man, Robert A.
Peppelenbosch, Maikel P.
Pan, Qiuwei
author_facet Li, Yunlong
Miao, Zhijiang
Li, Pengfei
Zhang, Ruyi
Kainov, Denis E.
Ma, Zhongren
de Man, Robert A.
Peppelenbosch, Maikel P.
Pan, Qiuwei
author_sort Li, Yunlong
collection PubMed
description We show that ivermectin, an FDA-approved anti-parasitic drug, effectively inhibits infection with hepatitis E virus (HEV) genotypes 1 and 3 in a range of cell culture models, including hepatic and extrahepatic cells. Long-term treatment showed no clear evidence of the development of drug resistance. Gene silencing of importin-α1, a cellular target of ivermectin and a key member of the host nuclear transport complex, inhibited viral replication and largely abolished the anti-HEV effect of ivermectin. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05096-w.
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spelling pubmed-81192332021-05-14 Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1 Li, Yunlong Miao, Zhijiang Li, Pengfei Zhang, Ruyi Kainov, Denis E. Ma, Zhongren de Man, Robert A. Peppelenbosch, Maikel P. Pan, Qiuwei Arch Virol Brief Report We show that ivermectin, an FDA-approved anti-parasitic drug, effectively inhibits infection with hepatitis E virus (HEV) genotypes 1 and 3 in a range of cell culture models, including hepatic and extrahepatic cells. Long-term treatment showed no clear evidence of the development of drug resistance. Gene silencing of importin-α1, a cellular target of ivermectin and a key member of the host nuclear transport complex, inhibited viral replication and largely abolished the anti-HEV effect of ivermectin. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05096-w. Springer Vienna 2021-05-14 2021 /pmc/articles/PMC8119233/ /pubmed/33987753 http://dx.doi.org/10.1007/s00705-021-05096-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Li, Yunlong
Miao, Zhijiang
Li, Pengfei
Zhang, Ruyi
Kainov, Denis E.
Ma, Zhongren
de Man, Robert A.
Peppelenbosch, Maikel P.
Pan, Qiuwei
Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1
title Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1
title_full Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1
title_fullStr Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1
title_full_unstemmed Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1
title_short Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1
title_sort ivermectin effectively inhibits hepatitis e virus replication, requiring the host nuclear transport protein importin α1
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119233/
https://www.ncbi.nlm.nih.gov/pubmed/33987753
http://dx.doi.org/10.1007/s00705-021-05096-w
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