Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis

Transgenes that are stably expressed in plant genomes over many generations could be assumed to behave epigenetically the same as endogenous genes. Here, we report that whereas the histone H3K9me2 demethylase IBM1, but not the histone H3K4me3 demethylase JMJ14, counteracts DNA methylation of Arabido...

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Autores principales: Butel, Nicolas, Yu, Agnès, Le Masson, Ivan, Borges, Filipe, Elmayan, Taline, Taochy, Christelle, Gursanscky, Nial R., Cao, Jiangling, Bi, Shengnan, Sawyer, Anne, Carroll, Bernard J., Vaucheret, Hervé
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119426/
https://www.ncbi.nlm.nih.gov/pubmed/33986281
http://dx.doi.org/10.1038/s41467-021-22995-3
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author Butel, Nicolas
Yu, Agnès
Le Masson, Ivan
Borges, Filipe
Elmayan, Taline
Taochy, Christelle
Gursanscky, Nial R.
Cao, Jiangling
Bi, Shengnan
Sawyer, Anne
Carroll, Bernard J.
Vaucheret, Hervé
author_facet Butel, Nicolas
Yu, Agnès
Le Masson, Ivan
Borges, Filipe
Elmayan, Taline
Taochy, Christelle
Gursanscky, Nial R.
Cao, Jiangling
Bi, Shengnan
Sawyer, Anne
Carroll, Bernard J.
Vaucheret, Hervé
author_sort Butel, Nicolas
collection PubMed
description Transgenes that are stably expressed in plant genomes over many generations could be assumed to behave epigenetically the same as endogenous genes. Here, we report that whereas the histone H3K9me2 demethylase IBM1, but not the histone H3K4me3 demethylase JMJ14, counteracts DNA methylation of Arabidopsis endogenous genes, JMJ14, but not IBM1, counteracts DNA methylation of expressed transgenes. Additionally, JMJ14-mediated specific attenuation of transgene DNA methylation enhances the production of aberrant RNAs that readily induce systemic post-transcriptional transgene silencing (PTGS). Thus, the JMJ14 chromatin modifying complex maintains expressed transgenes in a probationary state of susceptibility to PTGS, suggesting that the host plant genome does not immediately accept expressed transgenes as being epigenetically the same as endogenous genes.
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spelling pubmed-81194262021-05-14 Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis Butel, Nicolas Yu, Agnès Le Masson, Ivan Borges, Filipe Elmayan, Taline Taochy, Christelle Gursanscky, Nial R. Cao, Jiangling Bi, Shengnan Sawyer, Anne Carroll, Bernard J. Vaucheret, Hervé Nat Commun Article Transgenes that are stably expressed in plant genomes over many generations could be assumed to behave epigenetically the same as endogenous genes. Here, we report that whereas the histone H3K9me2 demethylase IBM1, but not the histone H3K4me3 demethylase JMJ14, counteracts DNA methylation of Arabidopsis endogenous genes, JMJ14, but not IBM1, counteracts DNA methylation of expressed transgenes. Additionally, JMJ14-mediated specific attenuation of transgene DNA methylation enhances the production of aberrant RNAs that readily induce systemic post-transcriptional transgene silencing (PTGS). Thus, the JMJ14 chromatin modifying complex maintains expressed transgenes in a probationary state of susceptibility to PTGS, suggesting that the host plant genome does not immediately accept expressed transgenes as being epigenetically the same as endogenous genes. Nature Publishing Group UK 2021-05-13 /pmc/articles/PMC8119426/ /pubmed/33986281 http://dx.doi.org/10.1038/s41467-021-22995-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Butel, Nicolas
Yu, Agnès
Le Masson, Ivan
Borges, Filipe
Elmayan, Taline
Taochy, Christelle
Gursanscky, Nial R.
Cao, Jiangling
Bi, Shengnan
Sawyer, Anne
Carroll, Bernard J.
Vaucheret, Hervé
Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis
title Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis
title_full Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis
title_fullStr Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis
title_full_unstemmed Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis
title_short Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis
title_sort contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119426/
https://www.ncbi.nlm.nih.gov/pubmed/33986281
http://dx.doi.org/10.1038/s41467-021-22995-3
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