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Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

A large number of insect-specific viruses (ISVs) have recently been discovered, mostly from hematophagous insect vectors because of their medical importance, but little attention has been paid to important plant virus vectors such as the whitefly Bemisia tabaci, which exists as a complex of cryptic...

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Autores principales: Huang, Hai-Jian, Ye, Zhuang-Xin, Wang, Xin, Yan, Xiao-Tian, Zhang, Yan, He, Yu-Juan, Qi, Yu-Hua, Zhang, Xiao-Di, Zhuo, Ji-Chong, Lu, Gang, Lu, Jia-Bao, Mao, Qian-Zhuo, Sun, Zong-Tao, Yan, Fei, Chen, Jian-Ping, Zhang, Chuan-Xi, Li, Jun-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119434/
https://www.ncbi.nlm.nih.gov/pubmed/33986295
http://dx.doi.org/10.1038/s41522-021-00216-5
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author Huang, Hai-Jian
Ye, Zhuang-Xin
Wang, Xin
Yan, Xiao-Tian
Zhang, Yan
He, Yu-Juan
Qi, Yu-Hua
Zhang, Xiao-Di
Zhuo, Ji-Chong
Lu, Gang
Lu, Jia-Bao
Mao, Qian-Zhuo
Sun, Zong-Tao
Yan, Fei
Chen, Jian-Ping
Zhang, Chuan-Xi
Li, Jun-Min
author_facet Huang, Hai-Jian
Ye, Zhuang-Xin
Wang, Xin
Yan, Xiao-Tian
Zhang, Yan
He, Yu-Juan
Qi, Yu-Hua
Zhang, Xiao-Di
Zhuo, Ji-Chong
Lu, Gang
Lu, Jia-Bao
Mao, Qian-Zhuo
Sun, Zong-Tao
Yan, Fei
Chen, Jian-Ping
Zhang, Chuan-Xi
Li, Jun-Min
author_sort Huang, Hai-Jian
collection PubMed
description A large number of insect-specific viruses (ISVs) have recently been discovered, mostly from hematophagous insect vectors because of their medical importance, but little attention has been paid to important plant virus vectors such as the whitefly Bemisia tabaci, which exists as a complex of cryptic species. Public SRA datasets of B. tabaci and newly generated transcriptomes of three Chinese populations are here comprehensively investigated to characterize the whitefly viromes of different cryptic species. Twenty novel ISVs were confidently identified, mostly associated with a particular cryptic species while different cryptic species harbored one or more core ISVs. Microinjection experiments showed that some ISVs might cross-infect between the two invasive whitefly cryptic species, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), but others appeared to have a more restricted host range, reflecting the possibility of distinct long-term coevolution of these ISVs and whitefly hosts. Moreover, analysis of the profiles of virus-derived small-interfering RNAs indicated that some of the ISVs can successfully replicate in whitefly and the antiviral RNAi pathway of B. tabaci is actively involved in response to ISV infections. Our study provides a comprehensive analysis of the RNA virome, the distinct relationships and cross-cryptic species infectivity of ISVs in an agriculturally important insect vector.
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spelling pubmed-81194342021-05-14 Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci Huang, Hai-Jian Ye, Zhuang-Xin Wang, Xin Yan, Xiao-Tian Zhang, Yan He, Yu-Juan Qi, Yu-Hua Zhang, Xiao-Di Zhuo, Ji-Chong Lu, Gang Lu, Jia-Bao Mao, Qian-Zhuo Sun, Zong-Tao Yan, Fei Chen, Jian-Ping Zhang, Chuan-Xi Li, Jun-Min NPJ Biofilms Microbiomes Article A large number of insect-specific viruses (ISVs) have recently been discovered, mostly from hematophagous insect vectors because of their medical importance, but little attention has been paid to important plant virus vectors such as the whitefly Bemisia tabaci, which exists as a complex of cryptic species. Public SRA datasets of B. tabaci and newly generated transcriptomes of three Chinese populations are here comprehensively investigated to characterize the whitefly viromes of different cryptic species. Twenty novel ISVs were confidently identified, mostly associated with a particular cryptic species while different cryptic species harbored one or more core ISVs. Microinjection experiments showed that some ISVs might cross-infect between the two invasive whitefly cryptic species, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), but others appeared to have a more restricted host range, reflecting the possibility of distinct long-term coevolution of these ISVs and whitefly hosts. Moreover, analysis of the profiles of virus-derived small-interfering RNAs indicated that some of the ISVs can successfully replicate in whitefly and the antiviral RNAi pathway of B. tabaci is actively involved in response to ISV infections. Our study provides a comprehensive analysis of the RNA virome, the distinct relationships and cross-cryptic species infectivity of ISVs in an agriculturally important insect vector. Nature Publishing Group UK 2021-05-13 /pmc/articles/PMC8119434/ /pubmed/33986295 http://dx.doi.org/10.1038/s41522-021-00216-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Huang, Hai-Jian
Ye, Zhuang-Xin
Wang, Xin
Yan, Xiao-Tian
Zhang, Yan
He, Yu-Juan
Qi, Yu-Hua
Zhang, Xiao-Di
Zhuo, Ji-Chong
Lu, Gang
Lu, Jia-Bao
Mao, Qian-Zhuo
Sun, Zong-Tao
Yan, Fei
Chen, Jian-Ping
Zhang, Chuan-Xi
Li, Jun-Min
Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_full Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_fullStr Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_full_unstemmed Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_short Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_sort diversity and infectivity of the rna virome among different cryptic species of an agriculturally important insect vector: whitefly bemisia tabaci
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119434/
https://www.ncbi.nlm.nih.gov/pubmed/33986295
http://dx.doi.org/10.1038/s41522-021-00216-5
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