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Functional regulation of an outer retina hyporeflective band on optical coherence tomography images

Human and animal retinal optical coherence tomography (OCT) images show a hyporeflective band (HB) between the photoreceptor tip and retinal pigment epithelium layers whose mechanisms are unclear. In mice, HB magnitude and the external limiting membrane-retinal pigment epithelium (ELM-RPE) thickness...

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Autores principales: Gao, Shasha, Li, Yichao, Bissig, David, Cohen, Ethan D., Podolsky, Robert H., Childers, Karen Lins, Vernon, Gregory, Chen, Sonia, Berkowitz, Bruce A., Qian, Haohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119672/
https://www.ncbi.nlm.nih.gov/pubmed/33986362
http://dx.doi.org/10.1038/s41598-021-89599-1
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author Gao, Shasha
Li, Yichao
Bissig, David
Cohen, Ethan D.
Podolsky, Robert H.
Childers, Karen Lins
Vernon, Gregory
Chen, Sonia
Berkowitz, Bruce A.
Qian, Haohua
author_facet Gao, Shasha
Li, Yichao
Bissig, David
Cohen, Ethan D.
Podolsky, Robert H.
Childers, Karen Lins
Vernon, Gregory
Chen, Sonia
Berkowitz, Bruce A.
Qian, Haohua
author_sort Gao, Shasha
collection PubMed
description Human and animal retinal optical coherence tomography (OCT) images show a hyporeflective band (HB) between the photoreceptor tip and retinal pigment epithelium layers whose mechanisms are unclear. In mice, HB magnitude and the external limiting membrane-retinal pigment epithelium (ELM-RPE) thickness appear to be dependent on light exposure, which is known to alter photoreceptor mitochondria respiration. Here, we test the hypothesis that these two OCT biomarkers are linked to metabolic activity of the retina. Acetazolamide, which acidifies the subretinal space, had no significant impact on HB magnitude but produced ELM-RPE thinning. Mitochondrial stimulation with 2,4-dinitrophenol reduced both HB magnitude and ELM-RPE thickness in parallel, and also reduced F-actin expression in the same retinal region, but without altering ERG responses. For mice strains with relatively lower (C57BL/6J) or higher (129S6/ev) rod mitochondrial efficacy, light-induced changes in HB magnitude and ELM-RPE thickness were correlated. Humans, analyzed from published data captured with a different protocol, showed a similar light–dark change pattern in HB magnitude as in the mice. Our results indicate that mitochondrial respiration underlies changes in HB magnitude upstream of the pH-sensitive ELM-RPE thickness response. These two distinct OCT biomarkers could be useful indices for non-invasively evaluating photoreceptor mitochondrial metabolic activity.
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spelling pubmed-81196722021-05-17 Functional regulation of an outer retina hyporeflective band on optical coherence tomography images Gao, Shasha Li, Yichao Bissig, David Cohen, Ethan D. Podolsky, Robert H. Childers, Karen Lins Vernon, Gregory Chen, Sonia Berkowitz, Bruce A. Qian, Haohua Sci Rep Article Human and animal retinal optical coherence tomography (OCT) images show a hyporeflective band (HB) between the photoreceptor tip and retinal pigment epithelium layers whose mechanisms are unclear. In mice, HB magnitude and the external limiting membrane-retinal pigment epithelium (ELM-RPE) thickness appear to be dependent on light exposure, which is known to alter photoreceptor mitochondria respiration. Here, we test the hypothesis that these two OCT biomarkers are linked to metabolic activity of the retina. Acetazolamide, which acidifies the subretinal space, had no significant impact on HB magnitude but produced ELM-RPE thinning. Mitochondrial stimulation with 2,4-dinitrophenol reduced both HB magnitude and ELM-RPE thickness in parallel, and also reduced F-actin expression in the same retinal region, but without altering ERG responses. For mice strains with relatively lower (C57BL/6J) or higher (129S6/ev) rod mitochondrial efficacy, light-induced changes in HB magnitude and ELM-RPE thickness were correlated. Humans, analyzed from published data captured with a different protocol, showed a similar light–dark change pattern in HB magnitude as in the mice. Our results indicate that mitochondrial respiration underlies changes in HB magnitude upstream of the pH-sensitive ELM-RPE thickness response. These two distinct OCT biomarkers could be useful indices for non-invasively evaluating photoreceptor mitochondrial metabolic activity. Nature Publishing Group UK 2021-05-13 /pmc/articles/PMC8119672/ /pubmed/33986362 http://dx.doi.org/10.1038/s41598-021-89599-1 Text en © The Author(s) 2021, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gao, Shasha
Li, Yichao
Bissig, David
Cohen, Ethan D.
Podolsky, Robert H.
Childers, Karen Lins
Vernon, Gregory
Chen, Sonia
Berkowitz, Bruce A.
Qian, Haohua
Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_full Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_fullStr Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_full_unstemmed Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_short Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_sort functional regulation of an outer retina hyporeflective band on optical coherence tomography images
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119672/
https://www.ncbi.nlm.nih.gov/pubmed/33986362
http://dx.doi.org/10.1038/s41598-021-89599-1
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