Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference

The Gram-positive Bacillus methanolicus shows plasmid-dependent methylotrophy. This facultative ribulose monophosphate (RuMP) cycle methylotroph possesses two fructose bisphosphate aldolases (FBA) with distinct kinetic properties. The chromosomally encoded FBA(C) is the major glycolytic aldolase. Th...

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Detalles Bibliográficos
Autores principales: Schultenkämper, Kerstin, Gütle, Desirée D., López, Marina Gil, Keller, Laura B., Zhang, Lin, Einsle, Oliver, Jacquot, Jean-Pierre, Wendisch, Volker F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119897/
https://www.ncbi.nlm.nih.gov/pubmed/33995334
http://dx.doi.org/10.3389/fmicb.2021.669220

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119897/
https://www.ncbi.nlm.nih.gov/pubmed/33995334
http://dx.doi.org/10.3389/fmicb.2021.669220

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