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Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production

An autoflocculating microalgal strain was isolated from coal mine effluent wastewater which was named as Scenedesmus sp. NC1 after morphological and molecularly characterization. Further analysis of internal transcribed spacer 2 (ITS2) and compensatory base changes (CBCs) showed it does not belong t...

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Autores principales: Kumar, Niwas, Banerjee, Chiranjib, Jagadevan, Sheeja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120858/
https://www.ncbi.nlm.nih.gov/pubmed/34026574
http://dx.doi.org/10.1016/j.btre.2021.e00621
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author Kumar, Niwas
Banerjee, Chiranjib
Jagadevan, Sheeja
author_facet Kumar, Niwas
Banerjee, Chiranjib
Jagadevan, Sheeja
author_sort Kumar, Niwas
collection PubMed
description An autoflocculating microalgal strain was isolated from coal mine effluent wastewater which was named as Scenedesmus sp. NC1 after morphological and molecularly characterization. Further analysis of internal transcribed spacer 2 (ITS2) and compensatory base changes (CBCs) showed it does not belong to the clade comprising Scenedesmus sensu stricto. In stationary phase of growth, Scenedesmus sp. NC1 exhibited excellent autoflocculation efficiency (> 88 %) within 150 min of setting. Temperature, pH, and inorganic metals exhibited minor influence on the autoflocculation activity of Scenedesmus sp. NC1. The fatty acid profiling of Scenedesmus sp.NC1 showed that palmitic acid (C16:0), oleic acid (C18:1), and stearic acid (18:0) accounted for more than 68 % of total fatty acids. Moreover, Scenedesmus sp. NC1 demonstrated significant bioflocculation potential over non-flocculating freshwater microalgae, Chlorella sp. NCQ and Micractinium sp. NCS2. Hence, Scenedesmus sp. NC1 could be effective for economical harvesting of other non-flocculating microalgae for productions of biodiesel and other metabolites.
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spelling pubmed-81208582021-05-20 Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production Kumar, Niwas Banerjee, Chiranjib Jagadevan, Sheeja Biotechnol Rep (Amst) Research Article An autoflocculating microalgal strain was isolated from coal mine effluent wastewater which was named as Scenedesmus sp. NC1 after morphological and molecularly characterization. Further analysis of internal transcribed spacer 2 (ITS2) and compensatory base changes (CBCs) showed it does not belong to the clade comprising Scenedesmus sensu stricto. In stationary phase of growth, Scenedesmus sp. NC1 exhibited excellent autoflocculation efficiency (> 88 %) within 150 min of setting. Temperature, pH, and inorganic metals exhibited minor influence on the autoflocculation activity of Scenedesmus sp. NC1. The fatty acid profiling of Scenedesmus sp.NC1 showed that palmitic acid (C16:0), oleic acid (C18:1), and stearic acid (18:0) accounted for more than 68 % of total fatty acids. Moreover, Scenedesmus sp. NC1 demonstrated significant bioflocculation potential over non-flocculating freshwater microalgae, Chlorella sp. NCQ and Micractinium sp. NCS2. Hence, Scenedesmus sp. NC1 could be effective for economical harvesting of other non-flocculating microalgae for productions of biodiesel and other metabolites. Elsevier 2021-04-24 /pmc/articles/PMC8120858/ /pubmed/34026574 http://dx.doi.org/10.1016/j.btre.2021.e00621 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Kumar, Niwas
Banerjee, Chiranjib
Jagadevan, Sheeja
Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production
title Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production
title_full Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production
title_fullStr Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production
title_full_unstemmed Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production
title_short Identification, characterization, and lipid profiling of microalgae Scenedesmus sp. NC1, isolated from coal mine effluent with potential for biofuel production
title_sort identification, characterization, and lipid profiling of microalgae scenedesmus sp. nc1, isolated from coal mine effluent with potential for biofuel production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120858/
https://www.ncbi.nlm.nih.gov/pubmed/34026574
http://dx.doi.org/10.1016/j.btre.2021.e00621
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