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De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)

OBJECTIVE: Insects are the most evolutionarily successful groups of organisms, and this success is largely due to their flight ability. Interestingly, some stick insects have lost their flight ability despite having wings. To elucidate the shift from wingless to flying forms during insect evolution,...

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Autores principales: Sakamoto, Takuma, Sasaki, Shunya, Yamaguchi, Nobuki, Nakano, Miho, Sato, Hiroki, Iwabuchi, Kikuo, Tabunoki, Hiroko, Simpson, Richard J., Bono, Hidemasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120901/
https://www.ncbi.nlm.nih.gov/pubmed/33985569
http://dx.doi.org/10.1186/s13104-021-05600-0
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author Sakamoto, Takuma
Sasaki, Shunya
Yamaguchi, Nobuki
Nakano, Miho
Sato, Hiroki
Iwabuchi, Kikuo
Tabunoki, Hiroko
Simpson, Richard J.
Bono, Hidemasa
author_facet Sakamoto, Takuma
Sasaki, Shunya
Yamaguchi, Nobuki
Nakano, Miho
Sato, Hiroki
Iwabuchi, Kikuo
Tabunoki, Hiroko
Simpson, Richard J.
Bono, Hidemasa
author_sort Sakamoto, Takuma
collection PubMed
description OBJECTIVE: Insects are the most evolutionarily successful groups of organisms, and this success is largely due to their flight ability. Interestingly, some stick insects have lost their flight ability despite having wings. To elucidate the shift from wingless to flying forms during insect evolution, we compared the nutritional metabolism system among flight-winged, flightless-winged, and flightless-wingless stick insect groups. RESULTS: Here, we report RNA sequencing of midgut transcriptome of Entoria okinawaensis, a prominent Japanese flightless-wingless stick insect, and the comparative analysis of its transcriptome in publicly available midgut transcriptomes obtained from seven stick insect species. A gene enrichment analysis for differentially expressed genes, including those obtained from winged vs wingless and flight vs flightless genes comparisons, revealed that carbohydrate metabolic process-related genes were highly expressed in the winged stick insect group. We also found that the expression of the mitochondrial enolase superfamily member 1 transcript was significantly higher in the winged stick insect group than in the wingless stick insect group. Our findings could indicate that carbohydrate metabolic processes are related to the evolutionary process through which stick insects gain the ability of flight. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05600-0.
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spelling pubmed-81209012021-05-17 De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea) Sakamoto, Takuma Sasaki, Shunya Yamaguchi, Nobuki Nakano, Miho Sato, Hiroki Iwabuchi, Kikuo Tabunoki, Hiroko Simpson, Richard J. Bono, Hidemasa BMC Res Notes Research Note OBJECTIVE: Insects are the most evolutionarily successful groups of organisms, and this success is largely due to their flight ability. Interestingly, some stick insects have lost their flight ability despite having wings. To elucidate the shift from wingless to flying forms during insect evolution, we compared the nutritional metabolism system among flight-winged, flightless-winged, and flightless-wingless stick insect groups. RESULTS: Here, we report RNA sequencing of midgut transcriptome of Entoria okinawaensis, a prominent Japanese flightless-wingless stick insect, and the comparative analysis of its transcriptome in publicly available midgut transcriptomes obtained from seven stick insect species. A gene enrichment analysis for differentially expressed genes, including those obtained from winged vs wingless and flight vs flightless genes comparisons, revealed that carbohydrate metabolic process-related genes were highly expressed in the winged stick insect group. We also found that the expression of the mitochondrial enolase superfamily member 1 transcript was significantly higher in the winged stick insect group than in the wingless stick insect group. Our findings could indicate that carbohydrate metabolic processes are related to the evolutionary process through which stick insects gain the ability of flight. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05600-0. BioMed Central 2021-05-13 /pmc/articles/PMC8120901/ /pubmed/33985569 http://dx.doi.org/10.1186/s13104-021-05600-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Sakamoto, Takuma
Sasaki, Shunya
Yamaguchi, Nobuki
Nakano, Miho
Sato, Hiroki
Iwabuchi, Kikuo
Tabunoki, Hiroko
Simpson, Richard J.
Bono, Hidemasa
De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)
title De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)
title_full De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)
title_fullStr De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)
title_full_unstemmed De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)
title_short De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)
title_sort de novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (phasmatodea)
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120901/
https://www.ncbi.nlm.nih.gov/pubmed/33985569
http://dx.doi.org/10.1186/s13104-021-05600-0
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