Cargando…

Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus

BACKGROUND: Idiopathic normal pressure hydrocephalus (iNPH) is a reversible CNS disease characterized by disturbed cerebrospinal fluid (CSF) dynamics. Changes in the extracellular matrix (ECM) composition might be involved in the pathophysiology of iNPH. The aim of this study was to explore possible...

Descripción completa

Detalles Bibliográficos
Autores principales: Minta, Karolina, Jeppsson, Anna, Brinkmalm, Gunnar, Portelius, Erik, Zetterberg, Henrik, Blennow, Kaj, Tullberg, Mats, Andreasson, Ulf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120927/
https://www.ncbi.nlm.nih.gov/pubmed/33985551
http://dx.doi.org/10.1186/s12987-021-00256-1
_version_ 1783692211455524864
author Minta, Karolina
Jeppsson, Anna
Brinkmalm, Gunnar
Portelius, Erik
Zetterberg, Henrik
Blennow, Kaj
Tullberg, Mats
Andreasson, Ulf
author_facet Minta, Karolina
Jeppsson, Anna
Brinkmalm, Gunnar
Portelius, Erik
Zetterberg, Henrik
Blennow, Kaj
Tullberg, Mats
Andreasson, Ulf
author_sort Minta, Karolina
collection PubMed
description BACKGROUND: Idiopathic normal pressure hydrocephalus (iNPH) is a reversible CNS disease characterized by disturbed cerebrospinal fluid (CSF) dynamics. Changes in the extracellular matrix (ECM) composition might be involved in the pathophysiology of iNPH. The aim of this study was to explore possible differences between lumbar and ventricular CSF concentrations of the ECM markers brevican and neurocan, matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-1 (TIMP-1) and their relation to clinical symptoms in iNPH patients before and after shunt surgery. METHODS: Paired lumbar and ventricular CSF was collected from 31 iNPH patients, before and four months after shunt surgery. CSF was analysed for concentrations of tryptic peptides originating from brevican and neurocan using a mass spectrometry-based panel, and for MMP-1, -2, -9, -10 and TIMP-1 using fluorescent or electrochemiluminescent immunoassays. RESULTS: Brevican and neurocan peptide levels were not influenced by CSF origin, but MMP-1, -2, -10 and TIMP-1 were increased (p ≤ 0.0005), and MMP-9 decreased (p ≤ 0.0003) in lumbar CSF compared with ventricular CSF. There was a general trend of ECM proteins to increase following shunt surgery. Ventricular TIMP-1 was inversely correlated with overall symptoms (rho = − 0.62, p < 0.0001). CSF concentrations of the majority of brevican and neurocan peptides were increased in iNPH patients with a history of cardiovascular disease (p ≤ 0.001, AUC = 0.84–0.94) compared with those without. CONCLUSION: Levels of the CNS-specific proteins brevican and neurocan did not differ between the lumbar and ventricular CSF, whereas the increase of several CNS-unspecific MMPs and TIMP-1 in lumbar CSF suggests contribution from peripheral tissues. The increase of ECM proteins in CSF following shunt surgery could indicate disturbed ECM dynamics in iNPH that are restored by restitution of CSF dynamics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12987-021-00256-1.
format Online
Article
Text
id pubmed-8120927
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-81209272021-05-17 Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus Minta, Karolina Jeppsson, Anna Brinkmalm, Gunnar Portelius, Erik Zetterberg, Henrik Blennow, Kaj Tullberg, Mats Andreasson, Ulf Fluids Barriers CNS Research BACKGROUND: Idiopathic normal pressure hydrocephalus (iNPH) is a reversible CNS disease characterized by disturbed cerebrospinal fluid (CSF) dynamics. Changes in the extracellular matrix (ECM) composition might be involved in the pathophysiology of iNPH. The aim of this study was to explore possible differences between lumbar and ventricular CSF concentrations of the ECM markers brevican and neurocan, matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-1 (TIMP-1) and their relation to clinical symptoms in iNPH patients before and after shunt surgery. METHODS: Paired lumbar and ventricular CSF was collected from 31 iNPH patients, before and four months after shunt surgery. CSF was analysed for concentrations of tryptic peptides originating from brevican and neurocan using a mass spectrometry-based panel, and for MMP-1, -2, -9, -10 and TIMP-1 using fluorescent or electrochemiluminescent immunoassays. RESULTS: Brevican and neurocan peptide levels were not influenced by CSF origin, but MMP-1, -2, -10 and TIMP-1 were increased (p ≤ 0.0005), and MMP-9 decreased (p ≤ 0.0003) in lumbar CSF compared with ventricular CSF. There was a general trend of ECM proteins to increase following shunt surgery. Ventricular TIMP-1 was inversely correlated with overall symptoms (rho = − 0.62, p < 0.0001). CSF concentrations of the majority of brevican and neurocan peptides were increased in iNPH patients with a history of cardiovascular disease (p ≤ 0.001, AUC = 0.84–0.94) compared with those without. CONCLUSION: Levels of the CNS-specific proteins brevican and neurocan did not differ between the lumbar and ventricular CSF, whereas the increase of several CNS-unspecific MMPs and TIMP-1 in lumbar CSF suggests contribution from peripheral tissues. The increase of ECM proteins in CSF following shunt surgery could indicate disturbed ECM dynamics in iNPH that are restored by restitution of CSF dynamics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12987-021-00256-1. BioMed Central 2021-05-13 /pmc/articles/PMC8120927/ /pubmed/33985551 http://dx.doi.org/10.1186/s12987-021-00256-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Minta, Karolina
Jeppsson, Anna
Brinkmalm, Gunnar
Portelius, Erik
Zetterberg, Henrik
Blennow, Kaj
Tullberg, Mats
Andreasson, Ulf
Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus
title Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus
title_full Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus
title_fullStr Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus
title_full_unstemmed Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus
title_short Lumbar and ventricular CSF concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus
title_sort lumbar and ventricular csf concentrations of extracellular matrix proteins before and after shunt surgery in idiopathic normal pressure hydrocephalus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120927/
https://www.ncbi.nlm.nih.gov/pubmed/33985551
http://dx.doi.org/10.1186/s12987-021-00256-1
work_keys_str_mv AT mintakarolina lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus
AT jeppssonanna lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus
AT brinkmalmgunnar lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus
AT porteliuserik lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus
AT zetterberghenrik lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus
AT blennowkaj lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus
AT tullbergmats lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus
AT andreassonulf lumbarandventricularcsfconcentrationsofextracellularmatrixproteinsbeforeandaftershuntsurgeryinidiopathicnormalpressurehydrocephalus