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Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis

Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie m...

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Autores principales: Arif, Muhammad, Islam, Saif ul, Alotaibi, Saqer S., Elshehawi, Ahmed M., A. Ahmed, Mohamed A., M. Al-Sadi, Abdullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121359/
https://www.ncbi.nlm.nih.gov/pubmed/33989327
http://dx.doi.org/10.1371/journal.pone.0251232
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author Arif, Muhammad
Islam, Saif ul
Alotaibi, Saqer S.
Elshehawi, Ahmed M.
A. Ahmed, Mohamed A.
M. Al-Sadi, Abdullah
author_facet Arif, Muhammad
Islam, Saif ul
Alotaibi, Saqer S.
Elshehawi, Ahmed M.
A. Ahmed, Mohamed A.
M. Al-Sadi, Abdullah
author_sort Arif, Muhammad
collection PubMed
description Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) were constructed by following novel protocol. All infectious clones were confirmed by cloning and sequencing. All of the infectious clones were agro-inoculated in Agrobacterium. After the agro-infiltrations, all clones were injected into Nicotiana benthamiana and jute plants under controlled condition. After 28 days of inoculation, plants exhibited typical symptoms of their corresponding viruses. All the symptomatic and asymptomatic leaves were collected from inoculated plants for further analysis. The southern blot analysis was used to confirm the infection of studied begomoviruses. At the end, all the products were sequenced and analyzed.
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spelling pubmed-81213592021-05-25 Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis Arif, Muhammad Islam, Saif ul Alotaibi, Saqer S. Elshehawi, Ahmed M. A. Ahmed, Mohamed A. M. Al-Sadi, Abdullah PLoS One Research Article Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) were constructed by following novel protocol. All infectious clones were confirmed by cloning and sequencing. All of the infectious clones were agro-inoculated in Agrobacterium. After the agro-infiltrations, all clones were injected into Nicotiana benthamiana and jute plants under controlled condition. After 28 days of inoculation, plants exhibited typical symptoms of their corresponding viruses. All the symptomatic and asymptomatic leaves were collected from inoculated plants for further analysis. The southern blot analysis was used to confirm the infection of studied begomoviruses. At the end, all the products were sequenced and analyzed. Public Library of Science 2021-05-14 /pmc/articles/PMC8121359/ /pubmed/33989327 http://dx.doi.org/10.1371/journal.pone.0251232 Text en © 2021 Arif et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Arif, Muhammad
Islam, Saif ul
Alotaibi, Saqer S.
Elshehawi, Ahmed M.
A. Ahmed, Mohamed A.
M. Al-Sadi, Abdullah
Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis
title Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis
title_full Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis
title_fullStr Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis
title_full_unstemmed Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis
title_short Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis
title_sort infectious clone construction and pathogenicity confirmation of cotton leaf curl multan virus (clcumuv), ramie mosaic virus (ramv) and corchorus yellow vein vietnam virus (coyvv) by southern blot analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121359/
https://www.ncbi.nlm.nih.gov/pubmed/33989327
http://dx.doi.org/10.1371/journal.pone.0251232
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