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Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes

While skeletal muscle has a high capacity for endogenous repair in acute injuries, volumetric muscle loss can leave long-lasting or permanent structural and functional deficits to the injured muscle and surrounding tissues. With clinical treatments failing to repair lost tissue, there is a great nee...

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Detalles Bibliográficos
Autores principales: Smoak, Mollie M., Hogan, Katie J., Grande-Allen, K. Jane, Mikos, Antonios G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121426/
https://www.ncbi.nlm.nih.gov/pubmed/33990336
http://dx.doi.org/10.1126/sciadv.abg4123
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author Smoak, Mollie M.
Hogan, Katie J.
Grande-Allen, K. Jane
Mikos, Antonios G.
author_facet Smoak, Mollie M.
Hogan, Katie J.
Grande-Allen, K. Jane
Mikos, Antonios G.
author_sort Smoak, Mollie M.
collection PubMed
description While skeletal muscle has a high capacity for endogenous repair in acute injuries, volumetric muscle loss can leave long-lasting or permanent structural and functional deficits to the injured muscle and surrounding tissues. With clinical treatments failing to repair lost tissue, there is a great need for a tissue-engineered therapy to promote skeletal muscle regeneration. In this study, we aim to assess the potential for electrospun decellularized skeletal muscle extracellular matrix (dECM) with tunable physicochemical properties to control mouse myoblast growth and myotube formation. The material properties as well as cell behavior – growth and differentiation – were assessed in response to modulation of crosslinking and scaffold architecture. The fabrication of a bioactive dECM-based system with tunable physicochemical properties that can control myotube formation has several applications in skeletal muscle engineering and may bring the field one step closer to developing a therapy to address these unmet clinical needs.
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spelling pubmed-81214262021-05-19 Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes Smoak, Mollie M. Hogan, Katie J. Grande-Allen, K. Jane Mikos, Antonios G. Sci Adv Research Articles While skeletal muscle has a high capacity for endogenous repair in acute injuries, volumetric muscle loss can leave long-lasting or permanent structural and functional deficits to the injured muscle and surrounding tissues. With clinical treatments failing to repair lost tissue, there is a great need for a tissue-engineered therapy to promote skeletal muscle regeneration. In this study, we aim to assess the potential for electrospun decellularized skeletal muscle extracellular matrix (dECM) with tunable physicochemical properties to control mouse myoblast growth and myotube formation. The material properties as well as cell behavior – growth and differentiation – were assessed in response to modulation of crosslinking and scaffold architecture. The fabrication of a bioactive dECM-based system with tunable physicochemical properties that can control myotube formation has several applications in skeletal muscle engineering and may bring the field one step closer to developing a therapy to address these unmet clinical needs. American Association for the Advancement of Science 2021-05-14 /pmc/articles/PMC8121426/ /pubmed/33990336 http://dx.doi.org/10.1126/sciadv.abg4123 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Smoak, Mollie M.
Hogan, Katie J.
Grande-Allen, K. Jane
Mikos, Antonios G.
Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes
title Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes
title_full Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes
title_fullStr Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes
title_full_unstemmed Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes
title_short Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes
title_sort bioinspired electrospun decm scaffolds guide cell growth and control the formation of myotubes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121426/
https://www.ncbi.nlm.nih.gov/pubmed/33990336
http://dx.doi.org/10.1126/sciadv.abg4123
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