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Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs

A major goal of current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine efforts is to elicit antibody responses that confer protection. Mapping the epitope targets of the SARS-CoV-2 antibody response is critical for vaccine design, diagnostics, and development of therapeutics. H...

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Autores principales: Stoddard, Caitlin I., Galloway, Jared, Chu, Helen Y., Shipley, Mackenzie M., Sung, Kevin, Itell, Hannah L., Wolf, Caitlin R., Logue, Jennifer K., Magedson, Ariana, Garrett, Meghan E., Crawford, Katharine H.D., Laserson, Uri, Matsen, Frederick A., Overbaugh, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121454/
https://www.ncbi.nlm.nih.gov/pubmed/33991511
http://dx.doi.org/10.1016/j.celrep.2021.109164
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author Stoddard, Caitlin I.
Galloway, Jared
Chu, Helen Y.
Shipley, Mackenzie M.
Sung, Kevin
Itell, Hannah L.
Wolf, Caitlin R.
Logue, Jennifer K.
Magedson, Ariana
Garrett, Meghan E.
Crawford, Katharine H.D.
Laserson, Uri
Matsen, Frederick A.
Overbaugh, Julie
author_facet Stoddard, Caitlin I.
Galloway, Jared
Chu, Helen Y.
Shipley, Mackenzie M.
Sung, Kevin
Itell, Hannah L.
Wolf, Caitlin R.
Logue, Jennifer K.
Magedson, Ariana
Garrett, Meghan E.
Crawford, Katharine H.D.
Laserson, Uri
Matsen, Frederick A.
Overbaugh, Julie
author_sort Stoddard, Caitlin I.
collection PubMed
description A major goal of current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine efforts is to elicit antibody responses that confer protection. Mapping the epitope targets of the SARS-CoV-2 antibody response is critical for vaccine design, diagnostics, and development of therapeutics. Here, we develop a pan-coronavirus phage display library to map antibody binding sites at high resolution within the complete viral proteomes of all known human-infecting coronaviruses in patients with mild or moderate/severe coronavirus disease 2019 (COVID-19). We find that the majority of immune responses to SARS-CoV-2 are targeted to the spike protein, nucleocapsid, and ORF1ab and include sites of mutation in current variants of concern. Some epitopes are identified in the majority of samples, while others are rare, and we find variation in the number of epitopes targeted between individuals. We find low levels of SARS-CoV-2 cross-reactivity in individuals with no exposure to the virus and significant cross-reactivity with endemic human coronaviruses (CoVs) in convalescent sera from patients with COVID-19.
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spelling pubmed-81214542021-05-17 Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs Stoddard, Caitlin I. Galloway, Jared Chu, Helen Y. Shipley, Mackenzie M. Sung, Kevin Itell, Hannah L. Wolf, Caitlin R. Logue, Jennifer K. Magedson, Ariana Garrett, Meghan E. Crawford, Katharine H.D. Laserson, Uri Matsen, Frederick A. Overbaugh, Julie Cell Rep Article A major goal of current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine efforts is to elicit antibody responses that confer protection. Mapping the epitope targets of the SARS-CoV-2 antibody response is critical for vaccine design, diagnostics, and development of therapeutics. Here, we develop a pan-coronavirus phage display library to map antibody binding sites at high resolution within the complete viral proteomes of all known human-infecting coronaviruses in patients with mild or moderate/severe coronavirus disease 2019 (COVID-19). We find that the majority of immune responses to SARS-CoV-2 are targeted to the spike protein, nucleocapsid, and ORF1ab and include sites of mutation in current variants of concern. Some epitopes are identified in the majority of samples, while others are rare, and we find variation in the number of epitopes targeted between individuals. We find low levels of SARS-CoV-2 cross-reactivity in individuals with no exposure to the virus and significant cross-reactivity with endemic human coronaviruses (CoVs) in convalescent sera from patients with COVID-19. The Authors. 2021-05-25 2021-05-06 /pmc/articles/PMC8121454/ /pubmed/33991511 http://dx.doi.org/10.1016/j.celrep.2021.109164 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Stoddard, Caitlin I.
Galloway, Jared
Chu, Helen Y.
Shipley, Mackenzie M.
Sung, Kevin
Itell, Hannah L.
Wolf, Caitlin R.
Logue, Jennifer K.
Magedson, Ariana
Garrett, Meghan E.
Crawford, Katharine H.D.
Laserson, Uri
Matsen, Frederick A.
Overbaugh, Julie
Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs
title Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs
title_full Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs
title_fullStr Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs
title_full_unstemmed Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs
title_short Epitope profiling reveals binding signatures of SARS-CoV-2 immune response in natural infection and cross-reactivity with endemic human CoVs
title_sort epitope profiling reveals binding signatures of sars-cov-2 immune response in natural infection and cross-reactivity with endemic human covs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121454/
https://www.ncbi.nlm.nih.gov/pubmed/33991511
http://dx.doi.org/10.1016/j.celrep.2021.109164
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