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A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria

Intestinal microbiota is gaining increasing interest from researchers, and a series of studies proved that gut bacteria plays a significant role in various malignancies, especially in colorectal cancer (CRC). In this study, a cohort of 34 CRC patients (average age=65 years old), 26 young volunteers...

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Autores principales: Zhang, Yu-Kun, Zhang, Qian, Wang, Yu-Liuming, Zhang, Wei-Yuan, Hu, Han-Qing, Wu, Hong-Yu, Sheng, Xiang-Zong, Luo, Kang-Jia, Zhang, Hao, Wang, Meng, Huang, Rui, Wang, Gui-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121496/
https://www.ncbi.nlm.nih.gov/pubmed/33996615
http://dx.doi.org/10.3389/fcimb.2021.606490
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author Zhang, Yu-Kun
Zhang, Qian
Wang, Yu-Liuming
Zhang, Wei-Yuan
Hu, Han-Qing
Wu, Hong-Yu
Sheng, Xiang-Zong
Luo, Kang-Jia
Zhang, Hao
Wang, Meng
Huang, Rui
Wang, Gui-Yu
author_facet Zhang, Yu-Kun
Zhang, Qian
Wang, Yu-Liuming
Zhang, Wei-Yuan
Hu, Han-Qing
Wu, Hong-Yu
Sheng, Xiang-Zong
Luo, Kang-Jia
Zhang, Hao
Wang, Meng
Huang, Rui
Wang, Gui-Yu
author_sort Zhang, Yu-Kun
collection PubMed
description Intestinal microbiota is gaining increasing interest from researchers, and a series of studies proved that gut bacteria plays a significant role in various malignancies, especially in colorectal cancer (CRC). In this study, a cohort of 34 CRC patients (average age=65 years old), 26 young volunteers (below 30 years old), and 26 old volunteers (over 60 years old) was enrolled. 16S ribosomal RNA gene sequencing was used to explore fecal bacteria diversity. The operational taxonomic unit (OTU) clustering analysis and NMDS (non-metric multidimensional scaling) analysis were used to separate different groups. Cluster of ortholog genes (COG) functional annotation and Kyoto encyclopedia of genes and genomes (KEGG) were used to detect enriched pathways among three groups. Community separations were observed among the three groups of this cohort. Clostridia, Actinobacteria, Bifidobacterium, and Fusobacteria were the most enriched bacteria in the young group, old group, and CRC group respectively. Also, in the young, old, and CRC group, the ratio of Firmicutes/Bacteroidetes was increased sequentially despite no statistical differences. Further, COG showed that transcription, cell wall/membrane/envelope biogenesis, inorganic ion transport and metabolism, and signal transduction mechanisms were differentially expressed among three groups. KEGG pathways associated with ABC transporters, amino sugar and nucleotide sugar metabolism, arginine and proline metabolism, and aminoacyl-tRNA biosynthesis also showed statistical differences among the three groups. These results indicated that the intestinal bacterial community varied as age changed and was related to CRC, and we discussed that specific bacteria enriched in the young and old group may exert a protective function, while bacteria enriched in the CRC group may promote tumorigenesis.
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spelling pubmed-81214962021-05-15 A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria Zhang, Yu-Kun Zhang, Qian Wang, Yu-Liuming Zhang, Wei-Yuan Hu, Han-Qing Wu, Hong-Yu Sheng, Xiang-Zong Luo, Kang-Jia Zhang, Hao Wang, Meng Huang, Rui Wang, Gui-Yu Front Cell Infect Microbiol Cellular and Infection Microbiology Intestinal microbiota is gaining increasing interest from researchers, and a series of studies proved that gut bacteria plays a significant role in various malignancies, especially in colorectal cancer (CRC). In this study, a cohort of 34 CRC patients (average age=65 years old), 26 young volunteers (below 30 years old), and 26 old volunteers (over 60 years old) was enrolled. 16S ribosomal RNA gene sequencing was used to explore fecal bacteria diversity. The operational taxonomic unit (OTU) clustering analysis and NMDS (non-metric multidimensional scaling) analysis were used to separate different groups. Cluster of ortholog genes (COG) functional annotation and Kyoto encyclopedia of genes and genomes (KEGG) were used to detect enriched pathways among three groups. Community separations were observed among the three groups of this cohort. Clostridia, Actinobacteria, Bifidobacterium, and Fusobacteria were the most enriched bacteria in the young group, old group, and CRC group respectively. Also, in the young, old, and CRC group, the ratio of Firmicutes/Bacteroidetes was increased sequentially despite no statistical differences. Further, COG showed that transcription, cell wall/membrane/envelope biogenesis, inorganic ion transport and metabolism, and signal transduction mechanisms were differentially expressed among three groups. KEGG pathways associated with ABC transporters, amino sugar and nucleotide sugar metabolism, arginine and proline metabolism, and aminoacyl-tRNA biosynthesis also showed statistical differences among the three groups. These results indicated that the intestinal bacterial community varied as age changed and was related to CRC, and we discussed that specific bacteria enriched in the young and old group may exert a protective function, while bacteria enriched in the CRC group may promote tumorigenesis. Frontiers Media S.A. 2021-04-30 /pmc/articles/PMC8121496/ /pubmed/33996615 http://dx.doi.org/10.3389/fcimb.2021.606490 Text en Copyright © 2021 Zhang, Zhang, Wang, Zhang, Hu, Wu, Sheng, Luo, Zhang, Wang, Huang and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Zhang, Yu-Kun
Zhang, Qian
Wang, Yu-Liuming
Zhang, Wei-Yuan
Hu, Han-Qing
Wu, Hong-Yu
Sheng, Xiang-Zong
Luo, Kang-Jia
Zhang, Hao
Wang, Meng
Huang, Rui
Wang, Gui-Yu
A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria
title A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria
title_full A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria
title_fullStr A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria
title_full_unstemmed A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria
title_short A Comparison Study of Age and Colorectal Cancer-Related Gut Bacteria
title_sort comparison study of age and colorectal cancer-related gut bacteria
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121496/
https://www.ncbi.nlm.nih.gov/pubmed/33996615
http://dx.doi.org/10.3389/fcimb.2021.606490
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