Protocol for mouse adult neural stem cell isolation and culture

This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell prope...

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Detalles Bibliográficos
Autores principales: Ahmed, Ahmed K.M.A., Isaksen, Toke Jost, Yamashita, Toshihide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121769/
https://www.ncbi.nlm.nih.gov/pubmed/34027481
http://dx.doi.org/10.1016/j.xpro.2021.100522
Descripción
Sumario:This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020).

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