A robust protocol for the generation of human midbrain organoids

The lack of advanced in vitro models recapitulating the human brain complexity is still a major obstacle in brain development and neurological disease research. Here, we describe a robust protocol to derive human midbrain organoids from neuroepithelial stem cells. These complex 3D models are charact...

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Detalles Bibliográficos
Autores principales: Zagare, Alise, Gobin, Matthieu, Monzel, Anna S., Schwamborn, Jens C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121770/
https://www.ncbi.nlm.nih.gov/pubmed/34027482
http://dx.doi.org/10.1016/j.xpro.2021.100524
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author Zagare, Alise
Gobin, Matthieu
Monzel, Anna S.
Schwamborn, Jens C.
author_facet Zagare, Alise
Gobin, Matthieu
Monzel, Anna S.
Schwamborn, Jens C.
author_sort Zagare, Alise
collection PubMed
description The lack of advanced in vitro models recapitulating the human brain complexity is still a major obstacle in brain development and neurological disease research. Here, we describe a robust protocol to derive human midbrain organoids from neuroepithelial stem cells. These complex 3D models are characterized by the presence of functional neurons, including dopaminergic neurons and glial cells, making them particularly attractive for the study of Parkinson disease. For complete details on the use and execution of this protocol, please refer to Monzel et al. (2017).
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spelling pubmed-81217702021-05-20 A robust protocol for the generation of human midbrain organoids Zagare, Alise Gobin, Matthieu Monzel, Anna S. Schwamborn, Jens C. STAR Protoc Protocol The lack of advanced in vitro models recapitulating the human brain complexity is still a major obstacle in brain development and neurological disease research. Here, we describe a robust protocol to derive human midbrain organoids from neuroepithelial stem cells. These complex 3D models are characterized by the presence of functional neurons, including dopaminergic neurons and glial cells, making them particularly attractive for the study of Parkinson disease. For complete details on the use and execution of this protocol, please refer to Monzel et al. (2017). Elsevier 2021-05-04 /pmc/articles/PMC8121770/ /pubmed/34027482 http://dx.doi.org/10.1016/j.xpro.2021.100524 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Zagare, Alise
Gobin, Matthieu
Monzel, Anna S.
Schwamborn, Jens C.
A robust protocol for the generation of human midbrain organoids
title A robust protocol for the generation of human midbrain organoids
title_full A robust protocol for the generation of human midbrain organoids
title_fullStr A robust protocol for the generation of human midbrain organoids
title_full_unstemmed A robust protocol for the generation of human midbrain organoids
title_short A robust protocol for the generation of human midbrain organoids
title_sort robust protocol for the generation of human midbrain organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121770/
https://www.ncbi.nlm.nih.gov/pubmed/34027482
http://dx.doi.org/10.1016/j.xpro.2021.100524
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