Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy

This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, na...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Yan, Wang, Fangfang, Wang, Haoyu, Bastians, Philipp, Hua, Yunfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121772/
https://www.ncbi.nlm.nih.gov/pubmed/34027478
http://dx.doi.org/10.1016/j.xpro.2021.100515
Descripción
Sumario:This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, nanometer-resolution three-dimensional imaging of subcellular structures in a targeted tonotopic range of the cochlea and enables fast volumetric scan at submicron resolution using a compact X-ray microscope. For complete details on the use and execution of this protocol, please refer to Hua et al. (2021).

Ejemplares similares