Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy

This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, na...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Yan, Wang, Fangfang, Wang, Haoyu, Bastians, Philipp, Hua, Yunfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121772/
https://www.ncbi.nlm.nih.gov/pubmed/34027478
http://dx.doi.org/10.1016/j.xpro.2021.100515
_version_ 1783692442385514496
author Lu, Yan
Wang, Fangfang
Wang, Haoyu
Bastians, Philipp
Hua, Yunfeng
author_facet Lu, Yan
Wang, Fangfang
Wang, Haoyu
Bastians, Philipp
Hua, Yunfeng
author_sort Lu, Yan
collection PubMed
description This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, nanometer-resolution three-dimensional imaging of subcellular structures in a targeted tonotopic range of the cochlea and enables fast volumetric scan at submicron resolution using a compact X-ray microscope. For complete details on the use and execution of this protocol, please refer to Hua et al. (2021).
format Online
Article
Text
id pubmed-8121772
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-81217722021-05-20 Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy Lu, Yan Wang, Fangfang Wang, Haoyu Bastians, Philipp Hua, Yunfeng STAR Protoc Protocol This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, nanometer-resolution three-dimensional imaging of subcellular structures in a targeted tonotopic range of the cochlea and enables fast volumetric scan at submicron resolution using a compact X-ray microscope. For complete details on the use and execution of this protocol, please refer to Hua et al. (2021). Elsevier 2021-05-04 /pmc/articles/PMC8121772/ /pubmed/34027478 http://dx.doi.org/10.1016/j.xpro.2021.100515 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lu, Yan
Wang, Fangfang
Wang, Haoyu
Bastians, Philipp
Hua, Yunfeng
Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_full Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_fullStr Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_full_unstemmed Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_short Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_sort large-scale 3d imaging of mouse cochlea using serial block-face scanning electron microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121772/
https://www.ncbi.nlm.nih.gov/pubmed/34027478
http://dx.doi.org/10.1016/j.xpro.2021.100515
work_keys_str_mv AT luyan largescale3dimagingofmousecochleausingserialblockfacescanningelectronmicroscopy
AT wangfangfang largescale3dimagingofmousecochleausingserialblockfacescanningelectronmicroscopy
AT wanghaoyu largescale3dimagingofmousecochleausingserialblockfacescanningelectronmicroscopy
AT bastiansphilipp largescale3dimagingofmousecochleausingserialblockfacescanningelectronmicroscopy
AT huayunfeng largescale3dimagingofmousecochleausingserialblockfacescanningelectronmicroscopy

Ejemplares similares