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Computational pipeline for designing guide RNAs for mismatch-CRISPRi

CRISPR interference is an increasingly popular method for perturbing gene expression. Guided by single-guide RNAs (sgRNAs), nuclease-deficient Cas9 proteins bind to specific DNA sequences and hinder transcription. Specificity is achieved through complementarity of the sgRNAs to the DNA. Changing com...

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Detalles Bibliográficos
Autores principales:	van Gestel, Jordi, Hawkins, John S., Todor, Horia, Gross, Carol A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121773/ https://www.ncbi.nlm.nih.gov/pubmed/34027480 http://dx.doi.org/10.1016/j.xpro.2021.100521

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