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3D particle averaging and detection of macromolecular symmetry in localization microscopy
Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particl...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121824/ https://www.ncbi.nlm.nih.gov/pubmed/33990554 http://dx.doi.org/10.1038/s41467-021-22006-5 |
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author | Heydarian, Hamidreza Joosten, Maarten Przybylski, Adrian Schueder, Florian Jungmann, Ralf Werkhoven, Ben van Keller-Findeisen, Jan Ries, Jonas Stallinga, Sjoerd Bates, Mark Rieger, Bernd |
author_facet | Heydarian, Hamidreza Joosten, Maarten Przybylski, Adrian Schueder, Florian Jungmann, Ralf Werkhoven, Ben van Keller-Findeisen, Jan Ries, Jonas Stallinga, Sjoerd Bates, Mark Rieger, Bernd |
author_sort | Heydarian, Hamidreza |
collection | PubMed |
description | Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particles. In this work, we present an approach for 3D single particle analysis in localization microscopy which hugely increases signal-to-noise ratio and resolution and enables determining the symmetry groups of macromolecular complexes. Our method does not require a structural template, and handles anisotropic localization uncertainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 and Nup107 subcomplexes of the nuclear pore complex acquired using multiple single molecule localization microscopy techniques, with their structural symmetry deducted from the data. |
format | Online Article Text |
id | pubmed-8121824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81218242021-05-18 3D particle averaging and detection of macromolecular symmetry in localization microscopy Heydarian, Hamidreza Joosten, Maarten Przybylski, Adrian Schueder, Florian Jungmann, Ralf Werkhoven, Ben van Keller-Findeisen, Jan Ries, Jonas Stallinga, Sjoerd Bates, Mark Rieger, Bernd Nat Commun Article Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particles. In this work, we present an approach for 3D single particle analysis in localization microscopy which hugely increases signal-to-noise ratio and resolution and enables determining the symmetry groups of macromolecular complexes. Our method does not require a structural template, and handles anisotropic localization uncertainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 and Nup107 subcomplexes of the nuclear pore complex acquired using multiple single molecule localization microscopy techniques, with their structural symmetry deducted from the data. Nature Publishing Group UK 2021-05-14 /pmc/articles/PMC8121824/ /pubmed/33990554 http://dx.doi.org/10.1038/s41467-021-22006-5 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Heydarian, Hamidreza Joosten, Maarten Przybylski, Adrian Schueder, Florian Jungmann, Ralf Werkhoven, Ben van Keller-Findeisen, Jan Ries, Jonas Stallinga, Sjoerd Bates, Mark Rieger, Bernd 3D particle averaging and detection of macromolecular symmetry in localization microscopy |
title | 3D particle averaging and detection of macromolecular symmetry in localization microscopy |
title_full | 3D particle averaging and detection of macromolecular symmetry in localization microscopy |
title_fullStr | 3D particle averaging and detection of macromolecular symmetry in localization microscopy |
title_full_unstemmed | 3D particle averaging and detection of macromolecular symmetry in localization microscopy |
title_short | 3D particle averaging and detection of macromolecular symmetry in localization microscopy |
title_sort | 3d particle averaging and detection of macromolecular symmetry in localization microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121824/ https://www.ncbi.nlm.nih.gov/pubmed/33990554 http://dx.doi.org/10.1038/s41467-021-22006-5 |
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