Isolation of adult mouse microglia using their in vitro adherent properties

Microglia are the primary innate immune effectors of the central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, the isolation of adult mouse microglia has proven more difficult. Here, we present a simple, widely accessible protocol to isolate pure m...

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Detalles Bibliográficos
Autores principales: Woolf, Zoe, Stevenson, Taylor J., Lee, Kevin, Jung, Yewon, Park, Thomas I.H., Curtis, Maurice A., Montgomery, Johanna M., Dragunow, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121984/
https://www.ncbi.nlm.nih.gov/pubmed/34027479
http://dx.doi.org/10.1016/j.xpro.2021.100518
Descripción
Sumario:Microglia are the primary innate immune effectors of the central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, the isolation of adult mouse microglia has proven more difficult. Here, we present a simple, widely accessible protocol to isolate pure microglia cultures from 4- to 14-month-old mouse brains using their adherent properties in vitro. These isolated microglia recapitulate the adherent properties of adult human microglia and present a more suitable model for studying age-related diseases. For complete details on the use and execution of this protocol in adult human microglia, please refer to Rustenhoven et al. (2016).

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