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Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images
Correlation of 3D images acquired on different microscopes can be a daunting prospect even for experienced users. This protocol describes steps for registration of images from soft X-ray absorption contrast imaging and super-resolution fluorescence imaging of hydrated biological materials at cryogen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121986/ https://www.ncbi.nlm.nih.gov/pubmed/34027487 http://dx.doi.org/10.1016/j.xpro.2021.100529 |
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author | Vyas, Nina Kunne, Stephan Fish, Thomas M. Dobbie, Ian M. Harkiolaki, Maria Paul-Gilloteaux, Perrine |
author_facet | Vyas, Nina Kunne, Stephan Fish, Thomas M. Dobbie, Ian M. Harkiolaki, Maria Paul-Gilloteaux, Perrine |
author_sort | Vyas, Nina |
collection | PubMed |
description | Correlation of 3D images acquired on different microscopes can be a daunting prospect even for experienced users. This protocol describes steps for registration of images from soft X-ray absorption contrast imaging and super-resolution fluorescence imaging of hydrated biological materials at cryogenic temperatures. Although it is developed for data generated at synchrotron beamlines that offer the above combination of microscopies, it is applicable to all analogous imaging systems where the same area of a sample is examined using successive non-destructive imaging techniques. For complete details on the use and execution of this protocol, please refer to Kounatidis et al. (2020). |
format | Online Article Text |
id | pubmed-8121986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81219862021-05-20 Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images Vyas, Nina Kunne, Stephan Fish, Thomas M. Dobbie, Ian M. Harkiolaki, Maria Paul-Gilloteaux, Perrine STAR Protoc Protocol Correlation of 3D images acquired on different microscopes can be a daunting prospect even for experienced users. This protocol describes steps for registration of images from soft X-ray absorption contrast imaging and super-resolution fluorescence imaging of hydrated biological materials at cryogenic temperatures. Although it is developed for data generated at synchrotron beamlines that offer the above combination of microscopies, it is applicable to all analogous imaging systems where the same area of a sample is examined using successive non-destructive imaging techniques. For complete details on the use and execution of this protocol, please refer to Kounatidis et al. (2020). Elsevier 2021-05-06 /pmc/articles/PMC8121986/ /pubmed/34027487 http://dx.doi.org/10.1016/j.xpro.2021.100529 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Vyas, Nina Kunne, Stephan Fish, Thomas M. Dobbie, Ian M. Harkiolaki, Maria Paul-Gilloteaux, Perrine Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images |
title | Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images |
title_full | Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images |
title_fullStr | Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images |
title_full_unstemmed | Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images |
title_short | Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images |
title_sort | protocol for image registration of correlative soft x-ray tomography and super-resolution structured illumination microscopy images |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121986/ https://www.ncbi.nlm.nih.gov/pubmed/34027487 http://dx.doi.org/10.1016/j.xpro.2021.100529 |
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