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Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images

Correlation of 3D images acquired on different microscopes can be a daunting prospect even for experienced users. This protocol describes steps for registration of images from soft X-ray absorption contrast imaging and super-resolution fluorescence imaging of hydrated biological materials at cryogen...

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Autores principales: Vyas, Nina, Kunne, Stephan, Fish, Thomas M., Dobbie, Ian M., Harkiolaki, Maria, Paul-Gilloteaux, Perrine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121986/
https://www.ncbi.nlm.nih.gov/pubmed/34027487
http://dx.doi.org/10.1016/j.xpro.2021.100529
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author Vyas, Nina
Kunne, Stephan
Fish, Thomas M.
Dobbie, Ian M.
Harkiolaki, Maria
Paul-Gilloteaux, Perrine
author_facet Vyas, Nina
Kunne, Stephan
Fish, Thomas M.
Dobbie, Ian M.
Harkiolaki, Maria
Paul-Gilloteaux, Perrine
author_sort Vyas, Nina
collection PubMed
description Correlation of 3D images acquired on different microscopes can be a daunting prospect even for experienced users. This protocol describes steps for registration of images from soft X-ray absorption contrast imaging and super-resolution fluorescence imaging of hydrated biological materials at cryogenic temperatures. Although it is developed for data generated at synchrotron beamlines that offer the above combination of microscopies, it is applicable to all analogous imaging systems where the same area of a sample is examined using successive non-destructive imaging techniques. For complete details on the use and execution of this protocol, please refer to Kounatidis et al. (2020).
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spelling pubmed-81219862021-05-20 Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images Vyas, Nina Kunne, Stephan Fish, Thomas M. Dobbie, Ian M. Harkiolaki, Maria Paul-Gilloteaux, Perrine STAR Protoc Protocol Correlation of 3D images acquired on different microscopes can be a daunting prospect even for experienced users. This protocol describes steps for registration of images from soft X-ray absorption contrast imaging and super-resolution fluorescence imaging of hydrated biological materials at cryogenic temperatures. Although it is developed for data generated at synchrotron beamlines that offer the above combination of microscopies, it is applicable to all analogous imaging systems where the same area of a sample is examined using successive non-destructive imaging techniques. For complete details on the use and execution of this protocol, please refer to Kounatidis et al. (2020). Elsevier 2021-05-06 /pmc/articles/PMC8121986/ /pubmed/34027487 http://dx.doi.org/10.1016/j.xpro.2021.100529 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Vyas, Nina
Kunne, Stephan
Fish, Thomas M.
Dobbie, Ian M.
Harkiolaki, Maria
Paul-Gilloteaux, Perrine
Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images
title Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images
title_full Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images
title_fullStr Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images
title_full_unstemmed Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images
title_short Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images
title_sort protocol for image registration of correlative soft x-ray tomography and super-resolution structured illumination microscopy images
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121986/
https://www.ncbi.nlm.nih.gov/pubmed/34027487
http://dx.doi.org/10.1016/j.xpro.2021.100529
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