A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices

Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, an...

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Detalles Bibliográficos
Autores principales: Leinders-Zufall, Trese, Storch, Ursula, Mederos y Schnitzler, Michael, Ojha, Navin K., Koike, Kohei, Gudermann, Thomas, Zufall, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121987/
https://www.ncbi.nlm.nih.gov/pubmed/34027485
http://dx.doi.org/10.1016/j.xpro.2021.100527
Descripción
Sumario:Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, and OptoDArG—for ultrarapid activation and deactivation of native TRPC2 channels in mouse vomeronasal sensory neurons and olfactory type B cells, as well as heterologously expressed human TRPC6 channels. Photoconversion can be achieved in mammalian tissue slices and enables all-optical stimulation and shutoff of TRPC channels. For complete details on the use and execution of this protocol, please refer to Leinders-Zufall et al. (2018).

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