A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices

Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, an...

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Autores principales: Leinders-Zufall, Trese, Storch, Ursula, Mederos y Schnitzler, Michael, Ojha, Navin K., Koike, Kohei, Gudermann, Thomas, Zufall, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121987/
https://www.ncbi.nlm.nih.gov/pubmed/34027485
http://dx.doi.org/10.1016/j.xpro.2021.100527
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author Leinders-Zufall, Trese
Storch, Ursula
Mederos y Schnitzler, Michael
Ojha, Navin K.
Koike, Kohei
Gudermann, Thomas
Zufall, Frank
author_facet Leinders-Zufall, Trese
Storch, Ursula
Mederos y Schnitzler, Michael
Ojha, Navin K.
Koike, Kohei
Gudermann, Thomas
Zufall, Frank
author_sort Leinders-Zufall, Trese
collection PubMed
description Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, and OptoDArG—for ultrarapid activation and deactivation of native TRPC2 channels in mouse vomeronasal sensory neurons and olfactory type B cells, as well as heterologously expressed human TRPC6 channels. Photoconversion can be achieved in mammalian tissue slices and enables all-optical stimulation and shutoff of TRPC channels. For complete details on the use and execution of this protocol, please refer to Leinders-Zufall et al. (2018).
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spelling pubmed-81219872021-05-20 A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices Leinders-Zufall, Trese Storch, Ursula Mederos y Schnitzler, Michael Ojha, Navin K. Koike, Kohei Gudermann, Thomas Zufall, Frank STAR Protoc Protocol Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, and OptoDArG—for ultrarapid activation and deactivation of native TRPC2 channels in mouse vomeronasal sensory neurons and olfactory type B cells, as well as heterologously expressed human TRPC6 channels. Photoconversion can be achieved in mammalian tissue slices and enables all-optical stimulation and shutoff of TRPC channels. For complete details on the use and execution of this protocol, please refer to Leinders-Zufall et al. (2018). Elsevier 2021-05-05 /pmc/articles/PMC8121987/ /pubmed/34027485 http://dx.doi.org/10.1016/j.xpro.2021.100527 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Leinders-Zufall, Trese
Storch, Ursula
Mederos y Schnitzler, Michael
Ojha, Navin K.
Koike, Kohei
Gudermann, Thomas
Zufall, Frank
A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
title A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
title_full A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
title_fullStr A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
title_full_unstemmed A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
title_short A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
title_sort diacylglycerol photoswitching protocol for studying trpc channel functions in mammalian cells and tissue slices
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121987/
https://www.ncbi.nlm.nih.gov/pubmed/34027485
http://dx.doi.org/10.1016/j.xpro.2021.100527
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