Cargando…

A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices

Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, an...

Descripción completa

Detalles Bibliográficos
Autores principales:	Leinders-Zufall, Trese, Storch, Ursula, Mederos y Schnitzler, Michael, Ojha, Navin K., Koike, Kohei, Gudermann, Thomas, Zufall, Frank
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121987/ https://www.ncbi.nlm.nih.gov/pubmed/34027485 http://dx.doi.org/10.1016/j.xpro.2021.100527

Ejemplares similares

BTDAzo: A Photoswitchable TRPC5 Channel Activator
por: Müller, Markus, et al.
Publicado: (2022)

Emerging Roles of Diacylglycerol-Sensitive TRPC4/5 Channels
por: Mederos y Schnitzler, Michael, et al.
Publicado: (2018)

Type 3 inositol 1,4,5-trisphosphate receptor is dispensable for sensory activation of the mammalian vomeronasal organ
por: Chamero, Pablo, et al.
Publicado: (2017)

Functional Overexpression of Vomeronasal Receptors Using a Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon
por: Stein, Benjamin, et al.
Publicado: (2016)

Ca(2+) Extrusion by NCX Is Compromised in Olfactory Sensory Neurons of OMP(−/−) Mice
por: Kwon, Hyun J., et al.
Publicado: (2009)

Sensing and avoiding sick conspecifics requires Gαi2(+) vomeronasal neurons
por: Weiss, Jan, et al.
Publicado: (2023)

Signaling mechanisms and behavioral function of the mouse basal vomeronasal neuroepithelium
por: Pérez-Gómez, Anabel, et al.
Publicado: (2014)

Lifespan extension in female mice by early, transient exposure to adult female olfactory cues
por: Garratt, Michael, et al.
Publicado: (2022)

A wide range of pheromone-stimulated sexual and reproductive behaviors in female mice depend on G protein Gαo
por: Oboti, Livio, et al.
Publicado: (2014)

Sensory Detection by the Vomeronasal Organ Modulates Experience-Dependent Social Behaviors in Female Mice
por: Trouillet, Anne-Charlotte, et al.
Publicado: (2021)

Newborn Interneurons in the Accessory Olfactory Bulb Promote Mate Recognition in Female Mice
por: Oboti, Livio, et al.
Publicado: (2011)

A Sensor for Low Environmental Oxygen in the Mouse Main Olfactory Epithelium
por: Bleymehl, Katherin, et al.
Publicado: (2016)

Diacylglycerol regulates acute hypoxic pulmonary vasoconstriction via TRPC6
por: Fuchs, Beate, et al.
Publicado: (2011)

Inhibition of Diacylglycerol–Sensitive TRPC Channels by Synthetic and Natural Steroids
por: Miehe, Susanne, et al.
Publicado: (2012)

Pregnancy and estrogen enhance neural progenitor-cell proliferation in the vomeronasal sensory epithelium
por: Oboti, Livio, et al.
Publicado: (2015)

Pharmacological Modulation of Diacylglycerol-Sensitive TRPC3/6/7 Channels
por: Harteneck, Christian, et al.
Publicado: (2011)

Activation of TRPC6 channels is essential for lung ischaemia–reperfusion induced oedema in mice
por: Weissmann, Norbert, et al.
Publicado: (2012)

Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour
por: Bufe, Bernd, et al.
Publicado: (2019)

Dynamic monitoring of G(i/o)-protein-mediated decreases of intracellular cAMP by FRET-based Epac sensors
por: Storch, Ursula, et al.
Publicado: (2017)

Receptor guanylyl cyclases in mammalian olfaction: from genes to function
por: Zufall, Frank
Publicado: (2009)

Classical Transient Receptor Potential 1 (TRPC1): Channel or Channel Regulator?
por: Dietrich, Alexander, et al.
Publicado: (2014)

The normalized slope conductance as a tool for quantitative analysis of current-voltage relations
por: Hermann, Christian, et al.
Publicado: (2022)

Diacylglycerols interact with the L2 lipidation site in TRPC3 to induce a sensitized channel state
por: Erkan‐Candag, Hazel, et al.
Publicado: (2022)

A succinate/SUCNR1-brush cell defense program in the tracheal epithelium
por: Perniss, Alexander, et al.
Publicado: (2023)

An engineered photoswitchable mammalian pyruvate kinase
por: Gehrig, Stefanie, et al.
Publicado: (2017)

Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse
por: Chitirala, Praneeth, et al.
Publicado: (2020)

Lipin-1-derived diacylglycerol activates intracellular TRPC3 which is critical for inflammatory signaling
por: Casas, Javier, et al.
Publicado: (2021)

Helix 8 is the essential structural motif of mechanosensitive GPCRs
por: Erdogmus, Serap, et al.
Publicado: (2019)

Organization and Plasticity of Sodium Channel Expression in the Mouse Olfactory and Vomeronasal Epithelia
por: Bolz, Florian, et al.
Publicado: (2017)

TRPC Channels in Cardiac Plasticity
por: Numaga-Tomita, Takuro, et al.
Publicado: (2020)

TRPC Channels in the SOCE Scenario
por: Lopez, Jose J., et al.
Publicado: (2020)

Corticolimbic Expression of TRPC4 and TRPC5 Channels in the Rodent Brain
por: Fowler, Melissa A., et al.
Publicado: (2007)

Munc13 mediates klotho-inhibitable diacylglycerol-stimulated exocytotic insertion of pre-docked TRPC6 vesicles
por: Xie, Jian, et al.
Publicado: (2020)

Novel Targets for Stroke Therapy: Special Focus on TRPC Channels and TRPC6
por: Liu, Lu, et al.
Publicado: (2020)

A photoswitchable GABA receptor channel blocker
por: Maleeva, Galyna, et al.
Publicado: (2019)

Store-operated Ca(2+) entry in primary murine lung fibroblasts is independent of classical transient receptor potential (TRPC) channels and contributes to cell migration
por: Bendiks, Larissa, et al.
Publicado: (2020)

TRPC channels in exercise-mimetic therapy
por: Numaga-Tomita, Takuro, et al.
Publicado: (2018)

TRPC3 Channels in Cardiac Fibrosis
por: Numaga-Tomita, Takuro, et al.
Publicado: (2017)

TRPC Channels in Proteinuric Kidney Diseases
por: Hall, Gentzon, et al.
Publicado: (2019)

Diverse modes of reproduction in the marine free-living ciliate Glauconema trihymene
por: Long, Hongan, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_t9lhnre9cmah5267ldh47eunf3