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Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121988/ https://www.ncbi.nlm.nih.gov/pubmed/34027486 http://dx.doi.org/10.1016/j.xpro.2021.100528 |
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author | Calvo-Garrido, Javier Winn, Dania Maffezzini, Camilla Wedell, Anna Freyer, Christoph Falk, Anna Wredenberg, Anna |
author_facet | Calvo-Garrido, Javier Winn, Dania Maffezzini, Camilla Wedell, Anna Freyer, Christoph Falk, Anna Wredenberg, Anna |
author_sort | Calvo-Garrido, Javier |
collection | PubMed |
description | Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012). |
format | Online Article Text |
id | pubmed-8121988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81219882021-05-20 Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro Calvo-Garrido, Javier Winn, Dania Maffezzini, Camilla Wedell, Anna Freyer, Christoph Falk, Anna Wredenberg, Anna STAR Protoc Protocol Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012). Elsevier 2021-05-06 /pmc/articles/PMC8121988/ /pubmed/34027486 http://dx.doi.org/10.1016/j.xpro.2021.100528 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Calvo-Garrido, Javier Winn, Dania Maffezzini, Camilla Wedell, Anna Freyer, Christoph Falk, Anna Wredenberg, Anna Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
title | Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
title_full | Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
title_fullStr | Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
title_full_unstemmed | Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
title_short | Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
title_sort | protocol for the derivation, culturing, and differentiation of human ips-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121988/ https://www.ncbi.nlm.nih.gov/pubmed/34027486 http://dx.doi.org/10.1016/j.xpro.2021.100528 |
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