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Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro

Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them....

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Autores principales: Calvo-Garrido, Javier, Winn, Dania, Maffezzini, Camilla, Wedell, Anna, Freyer, Christoph, Falk, Anna, Wredenberg, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121988/
https://www.ncbi.nlm.nih.gov/pubmed/34027486
http://dx.doi.org/10.1016/j.xpro.2021.100528
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author Calvo-Garrido, Javier
Winn, Dania
Maffezzini, Camilla
Wedell, Anna
Freyer, Christoph
Falk, Anna
Wredenberg, Anna
author_facet Calvo-Garrido, Javier
Winn, Dania
Maffezzini, Camilla
Wedell, Anna
Freyer, Christoph
Falk, Anna
Wredenberg, Anna
author_sort Calvo-Garrido, Javier
collection PubMed
description Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012).
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spelling pubmed-81219882021-05-20 Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro Calvo-Garrido, Javier Winn, Dania Maffezzini, Camilla Wedell, Anna Freyer, Christoph Falk, Anna Wredenberg, Anna STAR Protoc Protocol Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012). Elsevier 2021-05-06 /pmc/articles/PMC8121988/ /pubmed/34027486 http://dx.doi.org/10.1016/j.xpro.2021.100528 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Calvo-Garrido, Javier
Winn, Dania
Maffezzini, Camilla
Wedell, Anna
Freyer, Christoph
Falk, Anna
Wredenberg, Anna
Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
title Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
title_full Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
title_fullStr Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
title_full_unstemmed Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
title_short Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
title_sort protocol for the derivation, culturing, and differentiation of human ips-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121988/
https://www.ncbi.nlm.nih.gov/pubmed/34027486
http://dx.doi.org/10.1016/j.xpro.2021.100528
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