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Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications

Self-crosslinking of Tannic acid (TA) was accomplished to obtain poly(tannic acid) (p(TA)) particles in single step, surfactant free media using sodium periodate (NaIO(4)) as an oxidizing agent. Almost monodisperse p(TA) particles with 981 ± 76 nm sizes and −22 ± 4 mV zeta potential value with ellip...

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Autor principal: Sahiner, Nurettin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122271/
https://www.ncbi.nlm.nih.gov/pubmed/33921976
http://dx.doi.org/10.3390/molecules26092429
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author Sahiner, Nurettin
author_facet Sahiner, Nurettin
author_sort Sahiner, Nurettin
collection PubMed
description Self-crosslinking of Tannic acid (TA) was accomplished to obtain poly(tannic acid) (p(TA)) particles in single step, surfactant free media using sodium periodate (NaIO(4)) as an oxidizing agent. Almost monodisperse p(TA) particles with 981 ± 76 nm sizes and −22 ± 4 mV zeta potential value with ellipsoidal shape was obtained. Only slight degradation of p(TA) particles with 6.8 ± 0.2% was observed at pH 7.4 in PBS up to 15 days because of the irreversible covalent formation between TA units, suggesting that hydrolytic degradation is independent from the used amounts of oxidation agents. p(TA) particles were found to be non-hemolytic up to 0.5 mg/mL concentration and found not to affect blood clotting mechanism up to 2 mg/mL concentration. Antioxidant activity of p(TA) particles was investigated by total phenol content (TPC), ferric reducing antioxidant potential (FRAP), trolox equivalent antioxidant capacity (TEAC), total flavanoid content (TFC), and Fe (II) chelating activity. p(TA) particles showed strong antioxidant capability in comparison to TA molecules, except FRAP assay. The antibacterial activity of p(TA) particles was investigated by micro-dilution technique on E. coli as Gram‑negative and S. aureus as Gram-positive bacteria and found that p(TA) particles are more effective on S. aureus with over 50% inhibition at 20 mg/mL concentration attained.
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spelling pubmed-81222712021-05-16 Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications Sahiner, Nurettin Molecules Article Self-crosslinking of Tannic acid (TA) was accomplished to obtain poly(tannic acid) (p(TA)) particles in single step, surfactant free media using sodium periodate (NaIO(4)) as an oxidizing agent. Almost monodisperse p(TA) particles with 981 ± 76 nm sizes and −22 ± 4 mV zeta potential value with ellipsoidal shape was obtained. Only slight degradation of p(TA) particles with 6.8 ± 0.2% was observed at pH 7.4 in PBS up to 15 days because of the irreversible covalent formation between TA units, suggesting that hydrolytic degradation is independent from the used amounts of oxidation agents. p(TA) particles were found to be non-hemolytic up to 0.5 mg/mL concentration and found not to affect blood clotting mechanism up to 2 mg/mL concentration. Antioxidant activity of p(TA) particles was investigated by total phenol content (TPC), ferric reducing antioxidant potential (FRAP), trolox equivalent antioxidant capacity (TEAC), total flavanoid content (TFC), and Fe (II) chelating activity. p(TA) particles showed strong antioxidant capability in comparison to TA molecules, except FRAP assay. The antibacterial activity of p(TA) particles was investigated by micro-dilution technique on E. coli as Gram‑negative and S. aureus as Gram-positive bacteria and found that p(TA) particles are more effective on S. aureus with over 50% inhibition at 20 mg/mL concentration attained. MDPI 2021-04-22 /pmc/articles/PMC8122271/ /pubmed/33921976 http://dx.doi.org/10.3390/molecules26092429 Text en © 2021 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sahiner, Nurettin
Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications
title Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications
title_full Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications
title_fullStr Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications
title_full_unstemmed Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications
title_short Self-Crosslinked Ellipsoidal Poly(Tannic Acid) Particles for Bio-Medical Applications
title_sort self-crosslinked ellipsoidal poly(tannic acid) particles for bio-medical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122271/
https://www.ncbi.nlm.nih.gov/pubmed/33921976
http://dx.doi.org/10.3390/molecules26092429
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