Cargando…

Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis

Background: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. Methods: We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Aubry, Aurélien, Demey, Baptiste, François, Catherine, Duverlie, Gilles, Castelain, Sandrine, Helle, François, Brochot, Etienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122444/
https://www.ncbi.nlm.nih.gov/pubmed/33919328
http://dx.doi.org/10.3390/jcm10091815
_version_ 1783692619821350912
author Aubry, Aurélien
Demey, Baptiste
François, Catherine
Duverlie, Gilles
Castelain, Sandrine
Helle, François
Brochot, Etienne
author_facet Aubry, Aurélien
Demey, Baptiste
François, Catherine
Duverlie, Gilles
Castelain, Sandrine
Helle, François
Brochot, Etienne
author_sort Aubry, Aurélien
collection PubMed
description Background: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. Methods: We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT–PCR using several commercial assays. Results: Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays (p < 0.05). The percentage of patients with a neutralizing titer decreased from 82% before two months postdiagnosis to 57% after six months. This decrease appeared to be more marked for patients under 65 years old and those not requiring hospitalization. The percentage of serology reversion at 6 months was from 11% with the WANTAI total assay to over 39% with the ABBOTT IgG assay. The neutralizing antibody titers decreased in parallel with the decrease of total antibody titers, with important heterogeneity between assays. Conclusions: In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results.
format Online
Article
Text
id pubmed-8122444
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81224442021-05-16 Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis Aubry, Aurélien Demey, Baptiste François, Catherine Duverlie, Gilles Castelain, Sandrine Helle, François Brochot, Etienne J Clin Med Article Background: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. Methods: We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT–PCR using several commercial assays. Results: Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays (p < 0.05). The percentage of patients with a neutralizing titer decreased from 82% before two months postdiagnosis to 57% after six months. This decrease appeared to be more marked for patients under 65 years old and those not requiring hospitalization. The percentage of serology reversion at 6 months was from 11% with the WANTAI total assay to over 39% with the ABBOTT IgG assay. The neutralizing antibody titers decreased in parallel with the decrease of total antibody titers, with important heterogeneity between assays. Conclusions: In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results. MDPI 2021-04-21 /pmc/articles/PMC8122444/ /pubmed/33919328 http://dx.doi.org/10.3390/jcm10091815 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aubry, Aurélien
Demey, Baptiste
François, Catherine
Duverlie, Gilles
Castelain, Sandrine
Helle, François
Brochot, Etienne
Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis
title Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis
title_full Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis
title_fullStr Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis
title_full_unstemmed Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis
title_short Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis
title_sort longitudinal analysis and comparison of six serological assays up to eight months post-covid-19 diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122444/
https://www.ncbi.nlm.nih.gov/pubmed/33919328
http://dx.doi.org/10.3390/jcm10091815
work_keys_str_mv AT aubryaurelien longitudinalanalysisandcomparisonofsixserologicalassaysuptoeightmonthspostcovid19diagnosis
AT demeybaptiste longitudinalanalysisandcomparisonofsixserologicalassaysuptoeightmonthspostcovid19diagnosis
AT francoiscatherine longitudinalanalysisandcomparisonofsixserologicalassaysuptoeightmonthspostcovid19diagnosis
AT duverliegilles longitudinalanalysisandcomparisonofsixserologicalassaysuptoeightmonthspostcovid19diagnosis
AT castelainsandrine longitudinalanalysisandcomparisonofsixserologicalassaysuptoeightmonthspostcovid19diagnosis
AT hellefrancois longitudinalanalysisandcomparisonofsixserologicalassaysuptoeightmonthspostcovid19diagnosis
AT brochotetienne longitudinalanalysisandcomparisonofsixserologicalassaysuptoeightmonthspostcovid19diagnosis