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Barley Seeds miRNome Stability during Long-Term Storage and Aging

Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in v...

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Autores principales: Puchta, Marta, Groszyk, Jolanta, Małecka, Magdalena, Koter, Marek D., Niedzielski, Maciej, Rakoczy-Trojanowska, Monika, Boczkowska, Maja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122619/
https://www.ncbi.nlm.nih.gov/pubmed/33919202
http://dx.doi.org/10.3390/ijms22094315
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author Puchta, Marta
Groszyk, Jolanta
Małecka, Magdalena
Koter, Marek D.
Niedzielski, Maciej
Rakoczy-Trojanowska, Monika
Boczkowska, Maja
author_facet Puchta, Marta
Groszyk, Jolanta
Małecka, Magdalena
Koter, Marek D.
Niedzielski, Maciej
Rakoczy-Trojanowska, Monika
Boczkowska, Maja
author_sort Puchta, Marta
collection PubMed
description Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families, i.e., miR159, miR156, miR166, and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along with the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability as detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state.
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spelling pubmed-81226192021-05-16 Barley Seeds miRNome Stability during Long-Term Storage and Aging Puchta, Marta Groszyk, Jolanta Małecka, Magdalena Koter, Marek D. Niedzielski, Maciej Rakoczy-Trojanowska, Monika Boczkowska, Maja Int J Mol Sci Article Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families, i.e., miR159, miR156, miR166, and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along with the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability as detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state. MDPI 2021-04-21 /pmc/articles/PMC8122619/ /pubmed/33919202 http://dx.doi.org/10.3390/ijms22094315 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Puchta, Marta
Groszyk, Jolanta
Małecka, Magdalena
Koter, Marek D.
Niedzielski, Maciej
Rakoczy-Trojanowska, Monika
Boczkowska, Maja
Barley Seeds miRNome Stability during Long-Term Storage and Aging
title Barley Seeds miRNome Stability during Long-Term Storage and Aging
title_full Barley Seeds miRNome Stability during Long-Term Storage and Aging
title_fullStr Barley Seeds miRNome Stability during Long-Term Storage and Aging
title_full_unstemmed Barley Seeds miRNome Stability during Long-Term Storage and Aging
title_short Barley Seeds miRNome Stability during Long-Term Storage and Aging
title_sort barley seeds mirnome stability during long-term storage and aging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122619/
https://www.ncbi.nlm.nih.gov/pubmed/33919202
http://dx.doi.org/10.3390/ijms22094315
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