Cargando…
Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications
The increase in capabilities of Scanning Probe Microscopy (SPM) has resulted in a parallel increase in complexity that limits the use of this technique outside of specialised research laboratories. SPM automation could substantially expand its application domain, improve reproducibility and increase...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123492/ https://www.ncbi.nlm.nih.gov/pubmed/33925843 http://dx.doi.org/10.3390/s21093027 |
_version_ | 1783692922104840192 |
---|---|
author | Szeremeta, Witold K. Harniman, Robert L. Bermingham, Charlotte R. Antognozzi, Massimo |
author_facet | Szeremeta, Witold K. Harniman, Robert L. Bermingham, Charlotte R. Antognozzi, Massimo |
author_sort | Szeremeta, Witold K. |
collection | PubMed |
description | The increase in capabilities of Scanning Probe Microscopy (SPM) has resulted in a parallel increase in complexity that limits the use of this technique outside of specialised research laboratories. SPM automation could substantially expand its application domain, improve reproducibility and increase throughput. Here, we present a bottom-up design in which the combination of positioning stages, orientation, and detection of the probe produces an SPM design compatible with full automation. The resulting probe microscope achieves sub-femtonewton force sensitivity whilst preserving low mechanical drift ([Formula: see text] nm/min in-plane and [Formula: see text] nm/min vertically). The additional integration of total internal reflection microscopy, and the straightforward operations in liquid, make this instrument configuration particularly attractive to future biomedical applications. |
format | Online Article Text |
id | pubmed-8123492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81234922021-05-16 Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications Szeremeta, Witold K. Harniman, Robert L. Bermingham, Charlotte R. Antognozzi, Massimo Sensors (Basel) Article The increase in capabilities of Scanning Probe Microscopy (SPM) has resulted in a parallel increase in complexity that limits the use of this technique outside of specialised research laboratories. SPM automation could substantially expand its application domain, improve reproducibility and increase throughput. Here, we present a bottom-up design in which the combination of positioning stages, orientation, and detection of the probe produces an SPM design compatible with full automation. The resulting probe microscope achieves sub-femtonewton force sensitivity whilst preserving low mechanical drift ([Formula: see text] nm/min in-plane and [Formula: see text] nm/min vertically). The additional integration of total internal reflection microscopy, and the straightforward operations in liquid, make this instrument configuration particularly attractive to future biomedical applications. MDPI 2021-04-26 /pmc/articles/PMC8123492/ /pubmed/33925843 http://dx.doi.org/10.3390/s21093027 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Szeremeta, Witold K. Harniman, Robert L. Bermingham, Charlotte R. Antognozzi, Massimo Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications |
title | Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications |
title_full | Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications |
title_fullStr | Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications |
title_full_unstemmed | Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications |
title_short | Towards a Fully Automated Scanning Probe Microscope for Biomedical Applications |
title_sort | towards a fully automated scanning probe microscope for biomedical applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123492/ https://www.ncbi.nlm.nih.gov/pubmed/33925843 http://dx.doi.org/10.3390/s21093027 |
work_keys_str_mv | AT szeremetawitoldk towardsafullyautomatedscanningprobemicroscopeforbiomedicalapplications AT harnimanrobertl towardsafullyautomatedscanningprobemicroscopeforbiomedicalapplications AT berminghamcharlotter towardsafullyautomatedscanningprobemicroscopeforbiomedicalapplications AT antognozzimassimo towardsafullyautomatedscanningprobemicroscopeforbiomedicalapplications |