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Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123583/ https://www.ncbi.nlm.nih.gov/pubmed/33926137 http://dx.doi.org/10.3390/molecules26092534 |
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author | Treml, Jakub Večeřová, Petra Herczogová, Petra Šmejkal, Karel |
author_facet | Treml, Jakub Večeřová, Petra Herczogová, Petra Šmejkal, Karel |
author_sort | Treml, Jakub |
collection | PubMed |
description | Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays. |
format | Online Article Text |
id | pubmed-8123583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81235832021-05-16 Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays Treml, Jakub Večeřová, Petra Herczogová, Petra Šmejkal, Karel Molecules Article Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays. MDPI 2021-04-26 /pmc/articles/PMC8123583/ /pubmed/33926137 http://dx.doi.org/10.3390/molecules26092534 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Treml, Jakub Večeřová, Petra Herczogová, Petra Šmejkal, Karel Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays |
title | Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays |
title_full | Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays |
title_fullStr | Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays |
title_full_unstemmed | Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays |
title_short | Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays |
title_sort | direct and indirect antioxidant effects of selected plant phenolics in cell-based assays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123583/ https://www.ncbi.nlm.nih.gov/pubmed/33926137 http://dx.doi.org/10.3390/molecules26092534 |
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