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Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays

Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed...

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Autores principales: Treml, Jakub, Večeřová, Petra, Herczogová, Petra, Šmejkal, Karel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123583/
https://www.ncbi.nlm.nih.gov/pubmed/33926137
http://dx.doi.org/10.3390/molecules26092534
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author Treml, Jakub
Večeřová, Petra
Herczogová, Petra
Šmejkal, Karel
author_facet Treml, Jakub
Večeřová, Petra
Herczogová, Petra
Šmejkal, Karel
author_sort Treml, Jakub
collection PubMed
description Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays.
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spelling pubmed-81235832021-05-16 Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays Treml, Jakub Večeřová, Petra Herczogová, Petra Šmejkal, Karel Molecules Article Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays. MDPI 2021-04-26 /pmc/articles/PMC8123583/ /pubmed/33926137 http://dx.doi.org/10.3390/molecules26092534 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Treml, Jakub
Večeřová, Petra
Herczogová, Petra
Šmejkal, Karel
Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
title Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
title_full Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
title_fullStr Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
title_full_unstemmed Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
title_short Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
title_sort direct and indirect antioxidant effects of selected plant phenolics in cell-based assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123583/
https://www.ncbi.nlm.nih.gov/pubmed/33926137
http://dx.doi.org/10.3390/molecules26092534
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