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Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes

The protein eL38 is one of the smallest proteins of the mammalian ribosome, which is a component of its large (60S) subunit. The haploinsufficiency of eL38 in mice leads to the Tail-short mutant phenotype characterized by defects in the development of the axial skeleton caused by the poor translatio...

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Autores principales: Gopanenko, Alexander V., Kolobova, Alena V., Tupikin, Alexey E., Kabilov, Marsel R., Malygin, Alexey A., Karpova, Galina G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123606/
https://www.ncbi.nlm.nih.gov/pubmed/33926116
http://dx.doi.org/10.3390/ijms22094531
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author Gopanenko, Alexander V.
Kolobova, Alena V.
Tupikin, Alexey E.
Kabilov, Marsel R.
Malygin, Alexey A.
Karpova, Galina G.
author_facet Gopanenko, Alexander V.
Kolobova, Alena V.
Tupikin, Alexey E.
Kabilov, Marsel R.
Malygin, Alexey A.
Karpova, Galina G.
author_sort Gopanenko, Alexander V.
collection PubMed
description The protein eL38 is one of the smallest proteins of the mammalian ribosome, which is a component of its large (60S) subunit. The haploinsufficiency of eL38 in mice leads to the Tail-short mutant phenotype characterized by defects in the development of the axial skeleton caused by the poor translation of mRNA subsets of Hox genes. Using the ribosome profiling assay applied to HEK293 cells knocked down of eL38, we examined the effects of the lack of eL38 in 60S subunits on gene expression at the level of translation. A four-fold decrease in the cell content of eL38 was shown to result in significant changes in the translational efficiencies of 150 genes. Among the genes, whose expression at the level of translation was enhanced, there were mainly those associated with basic metabolic processes; namely, translation, protein folding, chromosome organization, splicing, and others. The set of genes with reduced translation efficiencies contained those that are mostly involved in the processes related to the regulation of transcription, including the activation of Hox genes. Thus, we demonstrated that eL38 insufficiency significantly affects the expression of certain genes at the translational level. Our findings facilitate understanding the possible causes of some anomalies in eL38-deficient animals.
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spelling pubmed-81236062021-05-16 Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes Gopanenko, Alexander V. Kolobova, Alena V. Tupikin, Alexey E. Kabilov, Marsel R. Malygin, Alexey A. Karpova, Galina G. Int J Mol Sci Article The protein eL38 is one of the smallest proteins of the mammalian ribosome, which is a component of its large (60S) subunit. The haploinsufficiency of eL38 in mice leads to the Tail-short mutant phenotype characterized by defects in the development of the axial skeleton caused by the poor translation of mRNA subsets of Hox genes. Using the ribosome profiling assay applied to HEK293 cells knocked down of eL38, we examined the effects of the lack of eL38 in 60S subunits on gene expression at the level of translation. A four-fold decrease in the cell content of eL38 was shown to result in significant changes in the translational efficiencies of 150 genes. Among the genes, whose expression at the level of translation was enhanced, there were mainly those associated with basic metabolic processes; namely, translation, protein folding, chromosome organization, splicing, and others. The set of genes with reduced translation efficiencies contained those that are mostly involved in the processes related to the regulation of transcription, including the activation of Hox genes. Thus, we demonstrated that eL38 insufficiency significantly affects the expression of certain genes at the translational level. Our findings facilitate understanding the possible causes of some anomalies in eL38-deficient animals. MDPI 2021-04-26 /pmc/articles/PMC8123606/ /pubmed/33926116 http://dx.doi.org/10.3390/ijms22094531 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gopanenko, Alexander V.
Kolobova, Alena V.
Tupikin, Alexey E.
Kabilov, Marsel R.
Malygin, Alexey A.
Karpova, Galina G.
Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes
title Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes
title_full Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes
title_fullStr Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes
title_full_unstemmed Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes
title_short Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes
title_sort knockdown of the ribosomal protein el38 in hek293 cells changes the translational efficiency of specific genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123606/
https://www.ncbi.nlm.nih.gov/pubmed/33926116
http://dx.doi.org/10.3390/ijms22094531
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