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Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress

This study aimed to identify sensitive and noninvasive biomarkers of early cyto-genotoxic, oxidative and inflammatory effects for exposure to volatile organic compounds (VOCs) in shipyard painters. On 17 (11 spray and 6 roller) painters (previously characterized for VOCs exposure to toluene, xylenes...

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Autores principales: Cavallo, Delia, Ursini, Cinzia Lucia, Fresegna, Anna Maria, Ciervo, Aureliano, Maiello, Raffaele, Buresti, Giuliana, Paci, Enrico, Pigini, Daniela, Gherardi, Monica, Carbonari, Damiano, Sisto, Renata, Tranfo, Giovanna, Iavicoli, Sergio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123868/
https://www.ncbi.nlm.nih.gov/pubmed/33925554
http://dx.doi.org/10.3390/ijerph18094645
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author Cavallo, Delia
Ursini, Cinzia Lucia
Fresegna, Anna Maria
Ciervo, Aureliano
Maiello, Raffaele
Buresti, Giuliana
Paci, Enrico
Pigini, Daniela
Gherardi, Monica
Carbonari, Damiano
Sisto, Renata
Tranfo, Giovanna
Iavicoli, Sergio
author_facet Cavallo, Delia
Ursini, Cinzia Lucia
Fresegna, Anna Maria
Ciervo, Aureliano
Maiello, Raffaele
Buresti, Giuliana
Paci, Enrico
Pigini, Daniela
Gherardi, Monica
Carbonari, Damiano
Sisto, Renata
Tranfo, Giovanna
Iavicoli, Sergio
author_sort Cavallo, Delia
collection PubMed
description This study aimed to identify sensitive and noninvasive biomarkers of early cyto-genotoxic, oxidative and inflammatory effects for exposure to volatile organic compounds (VOCs) in shipyard painters. On 17 (11 spray and 6 roller) painters (previously characterized for VOCs exposure to toluene, xylenes, ethylbenzene, ethyl acetate) and on 18 controls, we performed buccal micronucleus cytome (BMCyt) assay; Fpg-comet assay on lymphocytes; detection of urinary 8-oxoGua (8-oxo-7,8-dihydroguanine), 8-oxodGuo (8-oxo-7,8-dihydro-2′-deoxyguanosine) and 8-oxoGuo (8-oxo-7,8-dihydroguanosine), and cytokines release on serum. We found induction of cyto-genotoxicity by BMCyt assay and inflammatory effects (IL-6 and TNFα) in roller painters exposed to lower VOC concentrations than spray painters. In contrast, in both worker groups, we found direct and oxidative DNA damage by comet assay (with slightly higher oxidative DNA damage in roller) and significant increase of 8-oxoGuo and decrease of 8-oxodGuo and 8-oxoGua in respect to controls. The cyto-genotoxicity observed only on buccal cells of roller painters could be related to the task’s specificity and the different used protective equipment. Although limited by the small number of subjects, the study shows the usefulness of all the used biomarkers in the risk assessment of painters workers exposed to complex mixtures.
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spelling pubmed-81238682021-05-16 Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress Cavallo, Delia Ursini, Cinzia Lucia Fresegna, Anna Maria Ciervo, Aureliano Maiello, Raffaele Buresti, Giuliana Paci, Enrico Pigini, Daniela Gherardi, Monica Carbonari, Damiano Sisto, Renata Tranfo, Giovanna Iavicoli, Sergio Int J Environ Res Public Health Article This study aimed to identify sensitive and noninvasive biomarkers of early cyto-genotoxic, oxidative and inflammatory effects for exposure to volatile organic compounds (VOCs) in shipyard painters. On 17 (11 spray and 6 roller) painters (previously characterized for VOCs exposure to toluene, xylenes, ethylbenzene, ethyl acetate) and on 18 controls, we performed buccal micronucleus cytome (BMCyt) assay; Fpg-comet assay on lymphocytes; detection of urinary 8-oxoGua (8-oxo-7,8-dihydroguanine), 8-oxodGuo (8-oxo-7,8-dihydro-2′-deoxyguanosine) and 8-oxoGuo (8-oxo-7,8-dihydroguanosine), and cytokines release on serum. We found induction of cyto-genotoxicity by BMCyt assay and inflammatory effects (IL-6 and TNFα) in roller painters exposed to lower VOC concentrations than spray painters. In contrast, in both worker groups, we found direct and oxidative DNA damage by comet assay (with slightly higher oxidative DNA damage in roller) and significant increase of 8-oxoGuo and decrease of 8-oxodGuo and 8-oxoGua in respect to controls. The cyto-genotoxicity observed only on buccal cells of roller painters could be related to the task’s specificity and the different used protective equipment. Although limited by the small number of subjects, the study shows the usefulness of all the used biomarkers in the risk assessment of painters workers exposed to complex mixtures. MDPI 2021-04-27 /pmc/articles/PMC8123868/ /pubmed/33925554 http://dx.doi.org/10.3390/ijerph18094645 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cavallo, Delia
Ursini, Cinzia Lucia
Fresegna, Anna Maria
Ciervo, Aureliano
Maiello, Raffaele
Buresti, Giuliana
Paci, Enrico
Pigini, Daniela
Gherardi, Monica
Carbonari, Damiano
Sisto, Renata
Tranfo, Giovanna
Iavicoli, Sergio
Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress
title Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress
title_full Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress
title_fullStr Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress
title_full_unstemmed Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress
title_short Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress
title_sort occupational exposure in industrial painters: sensitive and noninvasive biomarkers to evaluate early cytotoxicity, genotoxicity and oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123868/
https://www.ncbi.nlm.nih.gov/pubmed/33925554
http://dx.doi.org/10.3390/ijerph18094645
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