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The effect of calcium palmitate on bacteria associated with infant gut microbiota

Gut microbiota development in formula‐fed and breast‐fed infants is known to differ. This could relate to the usage of unmodified vegetable oil instead of mammalian fat in infant formula (IF), causing the enhanced formation of the poorly soluble soap calcium palmitate (CP) in the infant's gut....

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Autores principales: Wang, Lu, Bravo‐Ruiseco, Gabriela, Happe, Randolph, He, Tao, van Dijl, Jan Maarten, Harmsen, Hermie J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123918/
https://www.ncbi.nlm.nih.gov/pubmed/34180592
http://dx.doi.org/10.1002/mbo3.1187
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author Wang, Lu
Bravo‐Ruiseco, Gabriela
Happe, Randolph
He, Tao
van Dijl, Jan Maarten
Harmsen, Hermie J. M.
author_facet Wang, Lu
Bravo‐Ruiseco, Gabriela
Happe, Randolph
He, Tao
van Dijl, Jan Maarten
Harmsen, Hermie J. M.
author_sort Wang, Lu
collection PubMed
description Gut microbiota development in formula‐fed and breast‐fed infants is known to differ. This could relate to the usage of unmodified vegetable oil instead of mammalian fat in infant formula (IF), causing the enhanced formation of the poorly soluble soap calcium palmitate (CP) in the infant's gut. Here we investigate in vitro the possible influence of CP on the infant gut bacteria. The growth of several bacterial species dominant in the infant's gut was analyzed by culturing in media with CP. Faecalibacterium prausnitzii as a sensitive representative was analyzed in detail by scanning transmission electron microscopy, membrane staining, gas chromatography, and microbial fuel cell experiments. Of all bacteria tested, the growth of several bifidobacteria and F. prausnitzii was reduced at 0.01 mg/ml CP, Bifidobacterium infantis stopped growing completely. CP reduced the cell envelope thickness of F. prausnitzii, disturbed the cell membrane fatty acids and function of membrane proteins involved in electron transport. CP inhibited the growth of bifidobacteria and faecalibacteria. This suggests that modification of fat in IF may benefit the development of the gut microbiota in formula‐fed infants by supporting the colonization of important beneficial bacteria in early life. Future clinical studies are needed to confirm this.
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spelling pubmed-81239182021-05-21 The effect of calcium palmitate on bacteria associated with infant gut microbiota Wang, Lu Bravo‐Ruiseco, Gabriela Happe, Randolph He, Tao van Dijl, Jan Maarten Harmsen, Hermie J. M. Microbiologyopen Original Articles Gut microbiota development in formula‐fed and breast‐fed infants is known to differ. This could relate to the usage of unmodified vegetable oil instead of mammalian fat in infant formula (IF), causing the enhanced formation of the poorly soluble soap calcium palmitate (CP) in the infant's gut. Here we investigate in vitro the possible influence of CP on the infant gut bacteria. The growth of several bacterial species dominant in the infant's gut was analyzed by culturing in media with CP. Faecalibacterium prausnitzii as a sensitive representative was analyzed in detail by scanning transmission electron microscopy, membrane staining, gas chromatography, and microbial fuel cell experiments. Of all bacteria tested, the growth of several bifidobacteria and F. prausnitzii was reduced at 0.01 mg/ml CP, Bifidobacterium infantis stopped growing completely. CP reduced the cell envelope thickness of F. prausnitzii, disturbed the cell membrane fatty acids and function of membrane proteins involved in electron transport. CP inhibited the growth of bifidobacteria and faecalibacteria. This suggests that modification of fat in IF may benefit the development of the gut microbiota in formula‐fed infants by supporting the colonization of important beneficial bacteria in early life. Future clinical studies are needed to confirm this. John Wiley and Sons Inc. 2021-05-15 /pmc/articles/PMC8123918/ /pubmed/34180592 http://dx.doi.org/10.1002/mbo3.1187 Text en © 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Lu
Bravo‐Ruiseco, Gabriela
Happe, Randolph
He, Tao
van Dijl, Jan Maarten
Harmsen, Hermie J. M.
The effect of calcium palmitate on bacteria associated with infant gut microbiota
title The effect of calcium palmitate on bacteria associated with infant gut microbiota
title_full The effect of calcium palmitate on bacteria associated with infant gut microbiota
title_fullStr The effect of calcium palmitate on bacteria associated with infant gut microbiota
title_full_unstemmed The effect of calcium palmitate on bacteria associated with infant gut microbiota
title_short The effect of calcium palmitate on bacteria associated with infant gut microbiota
title_sort effect of calcium palmitate on bacteria associated with infant gut microbiota
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123918/
https://www.ncbi.nlm.nih.gov/pubmed/34180592
http://dx.doi.org/10.1002/mbo3.1187
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