Cargando…

Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis

BACKGROUND: Gastric cancer (GC) is a common and deadly malignancy in the world. CircRNAs have emerged as important regulators in human diseases, including GC. In this work, we intended to explore the role of circ_CORO1C in GC progression and potential mechanism. METHODS: Quantitative real-time PCR (...

Descripción completa

Detalles Bibliográficos
Autores principales: Fan, Yongqiang, Liu, Min, Liu, Anquan, Cui, Nailing, Chen, Zhimei, Yang, Qian, Su, Aihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123969/
https://www.ncbi.nlm.nih.gov/pubmed/34007212
http://dx.doi.org/10.2147/CMAR.S290629
_version_ 1783693072687693824
author Fan, Yongqiang
Liu, Min
Liu, Anquan
Cui, Nailing
Chen, Zhimei
Yang, Qian
Su, Aihua
author_facet Fan, Yongqiang
Liu, Min
Liu, Anquan
Cui, Nailing
Chen, Zhimei
Yang, Qian
Su, Aihua
author_sort Fan, Yongqiang
collection PubMed
description BACKGROUND: Gastric cancer (GC) is a common and deadly malignancy in the world. CircRNAs have emerged as important regulators in human diseases, including GC. In this work, we intended to explore the role of circ_CORO1C in GC progression and potential mechanism. METHODS: Quantitative real-time PCR (qRT-PCR) or Western blot assay was performed to examine the expression of circRNA coronin-like actin-binding protein 1C (circ_CORO1C), microRNA (miR)-138-5p and Krueppel-like factor 12 (KLF12) in clinical samples and cells. Cell colony formation ability and viability were measured by colony formation assay and methyl thiazolyl tetrazolium (MTT) assay, respectively. Expression of cell proliferation and epithelia-mesenchymal transition (EMT) biomarker was detected by Western blot analysis. And cell metastasis, including migration and invasion, and apoptosis were analyzed via Transwell assay and flow cytometry, respectively. Target relationship among circ_CORO1C, miR-138-5p and KLF12 was validated by dual-luciferase reporter assay. The in vivo role of circ_CORO1C was investigated by tumor xenograft assay. RESULTS: Circ_CORO1C and KLF12 were upregulated, while miR-138-5p was downregulated in GC tissues and cells. Circ_CORO1C knockdown suppressed colony formation ability, viability, migration, invasion and EMT in GC cells, while promoted cell apoptosis in vitro. Circ_CORO1C targeted miR-138-5p, the inhibition of which could attenuate silenced circ_CORO1C-induced inhibitory effects on GC progression. MiR-138-5p repressed the aggressive malignant behaviors of GC cells by directly targeting KLF12. Circ_CORO1C deficiency inhibited GC tumor growth in vivo. CONCLUSION: Depletion of circ_CORO1C suppressed GC progression by regulating miR-138-5p/KLF12 axis, offering a potential molecular target for GC therapy.
format Online
Article
Text
id pubmed-8123969
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-81239692021-05-17 Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis Fan, Yongqiang Liu, Min Liu, Anquan Cui, Nailing Chen, Zhimei Yang, Qian Su, Aihua Cancer Manag Res Original Research BACKGROUND: Gastric cancer (GC) is a common and deadly malignancy in the world. CircRNAs have emerged as important regulators in human diseases, including GC. In this work, we intended to explore the role of circ_CORO1C in GC progression and potential mechanism. METHODS: Quantitative real-time PCR (qRT-PCR) or Western blot assay was performed to examine the expression of circRNA coronin-like actin-binding protein 1C (circ_CORO1C), microRNA (miR)-138-5p and Krueppel-like factor 12 (KLF12) in clinical samples and cells. Cell colony formation ability and viability were measured by colony formation assay and methyl thiazolyl tetrazolium (MTT) assay, respectively. Expression of cell proliferation and epithelia-mesenchymal transition (EMT) biomarker was detected by Western blot analysis. And cell metastasis, including migration and invasion, and apoptosis were analyzed via Transwell assay and flow cytometry, respectively. Target relationship among circ_CORO1C, miR-138-5p and KLF12 was validated by dual-luciferase reporter assay. The in vivo role of circ_CORO1C was investigated by tumor xenograft assay. RESULTS: Circ_CORO1C and KLF12 were upregulated, while miR-138-5p was downregulated in GC tissues and cells. Circ_CORO1C knockdown suppressed colony formation ability, viability, migration, invasion and EMT in GC cells, while promoted cell apoptosis in vitro. Circ_CORO1C targeted miR-138-5p, the inhibition of which could attenuate silenced circ_CORO1C-induced inhibitory effects on GC progression. MiR-138-5p repressed the aggressive malignant behaviors of GC cells by directly targeting KLF12. Circ_CORO1C deficiency inhibited GC tumor growth in vivo. CONCLUSION: Depletion of circ_CORO1C suppressed GC progression by regulating miR-138-5p/KLF12 axis, offering a potential molecular target for GC therapy. Dove 2021-05-11 /pmc/articles/PMC8123969/ /pubmed/34007212 http://dx.doi.org/10.2147/CMAR.S290629 Text en © 2021 Fan et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Fan, Yongqiang
Liu, Min
Liu, Anquan
Cui, Nailing
Chen, Zhimei
Yang, Qian
Su, Aihua
Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis
title Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis
title_full Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis
title_fullStr Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis
title_full_unstemmed Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis
title_short Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis
title_sort depletion of circular rna circ_coro1c suppresses gastric cancer development by modulating mir-138-5p/klf12 axis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123969/
https://www.ncbi.nlm.nih.gov/pubmed/34007212
http://dx.doi.org/10.2147/CMAR.S290629
work_keys_str_mv AT fanyongqiang depletionofcircularrnacirccoro1csuppressesgastriccancerdevelopmentbymodulatingmir1385pklf12axis
AT liumin depletionofcircularrnacirccoro1csuppressesgastriccancerdevelopmentbymodulatingmir1385pklf12axis
AT liuanquan depletionofcircularrnacirccoro1csuppressesgastriccancerdevelopmentbymodulatingmir1385pklf12axis
AT cuinailing depletionofcircularrnacirccoro1csuppressesgastriccancerdevelopmentbymodulatingmir1385pklf12axis
AT chenzhimei depletionofcircularrnacirccoro1csuppressesgastriccancerdevelopmentbymodulatingmir1385pklf12axis
AT yangqian depletionofcircularrnacirccoro1csuppressesgastriccancerdevelopmentbymodulatingmir1385pklf12axis
AT suaihua depletionofcircularrnacirccoro1csuppressesgastriccancerdevelopmentbymodulatingmir1385pklf12axis