Cargando…
Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway
AIM: The naturally fermented yak yogurt of pastoralists in the Tibetan Plateau, China, because of its unique geographical environment and the unique lifestyle of Tibetan pastoralists, is very different from other kinds of sour milk, and the microorganisms it contains are special. Lactococcus lactis...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123977/ https://www.ncbi.nlm.nih.gov/pubmed/34007157 http://dx.doi.org/10.2147/DDDT.S309675 |
_version_ | 1783693075109904384 |
---|---|
author | Tan, Qian Hu, Jing Zhou, Yujing Wan, Yunxiao Zhang, Chuanlan Liu, Xin Long, Xingyao Tan, Fang Zhao, Xin |
author_facet | Tan, Qian Hu, Jing Zhou, Yujing Wan, Yunxiao Zhang, Chuanlan Liu, Xin Long, Xingyao Tan, Fang Zhao, Xin |
author_sort | Tan, Qian |
collection | PubMed |
description | AIM: The naturally fermented yak yogurt of pastoralists in the Tibetan Plateau, China, because of its unique geographical environment and the unique lifestyle of Tibetan pastoralists, is very different from other kinds of sour milk, and the microorganisms it contains are special. Lactococcus lactis subsp. lactis HFY14 (LLSL-HFY14) is a new lactic acid bacterium isolated from naturally fermented yak yogurt. The purpose of this study was to study the inhibitory effect of the bacterium on constipation. METHODS: Constipation was induced in ICR mice with diphenoxylate, and the constipated mice were treated with LLSL-HFY14. The weight and feces of the mice were visually detected. Colonic tissues were observed on hematoxylin and eosin-stained sections. Serum indices were detected with kits. mRNA expression in the colon was determined by quantitative polymerase chain reaction assay. RESULTS: Constipation caused weight loss, the number of defecation granules, defecation weight, fecal water content decreased, and the first black stool excretion time increased. LLSL-HFY14 alleviated these symptoms, and the effects were similar to those of lactulose (drug). The pathological examination revealed that constipation caused pathological changes in the colon, and LLSL-HFY14 effectively alleviated the disease. LLSL-HFY14 increased serum levels of motilin, gastrin, endothelin, substance P, acetylcholinesterase, and vasoactive intestinal peptide (VIP) and decreased serum levels of somatostatin in constipated mice. In addition, LLSL-HFY14 upregulated VIP, cAMP, protein kinase A, and aquaporin 3 expression in colonic tissues of constipated mice in a dose-dependent manner. CONCLUSION: LLSL-HFY14 inhibited constipation, similar to lactulose, and has the potential to become a biological agent. |
format | Online Article Text |
id | pubmed-8123977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-81239772021-05-17 Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway Tan, Qian Hu, Jing Zhou, Yujing Wan, Yunxiao Zhang, Chuanlan Liu, Xin Long, Xingyao Tan, Fang Zhao, Xin Drug Des Devel Ther Original Research AIM: The naturally fermented yak yogurt of pastoralists in the Tibetan Plateau, China, because of its unique geographical environment and the unique lifestyle of Tibetan pastoralists, is very different from other kinds of sour milk, and the microorganisms it contains are special. Lactococcus lactis subsp. lactis HFY14 (LLSL-HFY14) is a new lactic acid bacterium isolated from naturally fermented yak yogurt. The purpose of this study was to study the inhibitory effect of the bacterium on constipation. METHODS: Constipation was induced in ICR mice with diphenoxylate, and the constipated mice were treated with LLSL-HFY14. The weight and feces of the mice were visually detected. Colonic tissues were observed on hematoxylin and eosin-stained sections. Serum indices were detected with kits. mRNA expression in the colon was determined by quantitative polymerase chain reaction assay. RESULTS: Constipation caused weight loss, the number of defecation granules, defecation weight, fecal water content decreased, and the first black stool excretion time increased. LLSL-HFY14 alleviated these symptoms, and the effects were similar to those of lactulose (drug). The pathological examination revealed that constipation caused pathological changes in the colon, and LLSL-HFY14 effectively alleviated the disease. LLSL-HFY14 increased serum levels of motilin, gastrin, endothelin, substance P, acetylcholinesterase, and vasoactive intestinal peptide (VIP) and decreased serum levels of somatostatin in constipated mice. In addition, LLSL-HFY14 upregulated VIP, cAMP, protein kinase A, and aquaporin 3 expression in colonic tissues of constipated mice in a dose-dependent manner. CONCLUSION: LLSL-HFY14 inhibited constipation, similar to lactulose, and has the potential to become a biological agent. Dove 2021-05-11 /pmc/articles/PMC8123977/ /pubmed/34007157 http://dx.doi.org/10.2147/DDDT.S309675 Text en © 2021 Tan et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Tan, Qian Hu, Jing Zhou, Yujing Wan, Yunxiao Zhang, Chuanlan Liu, Xin Long, Xingyao Tan, Fang Zhao, Xin Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway |
title | Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway |
title_full | Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway |
title_fullStr | Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway |
title_full_unstemmed | Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway |
title_short | Inhibitory Effect of Lactococcus lactis subsp. lactis HFY14 on Diphenoxylate-Induced Constipation in Mice by Regulating the VIP-cAMP-PKA-AQP3 Signaling Pathway |
title_sort | inhibitory effect of lactococcus lactis subsp. lactis hfy14 on diphenoxylate-induced constipation in mice by regulating the vip-camp-pka-aqp3 signaling pathway |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123977/ https://www.ncbi.nlm.nih.gov/pubmed/34007157 http://dx.doi.org/10.2147/DDDT.S309675 |
work_keys_str_mv | AT tanqian inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT hujing inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT zhouyujing inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT wanyunxiao inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT zhangchuanlan inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT liuxin inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT longxingyao inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT tanfang inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway AT zhaoxin inhibitoryeffectoflactococcuslactissubsplactishfy14ondiphenoxylateinducedconstipationinmicebyregulatingthevipcamppkaaqp3signalingpathway |