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Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties

To the present day, no efficient plant protection method against economically important bacterial phytopathogens from the Pectobacteriaceae family has been implemented into agricultural practice. In this view, we have performed a multivariate optimization of the operating parameters of the reaction-...

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Autores principales: Dzimitrowicz, Anna, Jamroz, Piotr, Pohl, Pawel, Babinska, Weronika, Terefinko, Dominik, Sledz, Wojciech, Motyka-Pomagruk, Agata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124219/
https://www.ncbi.nlm.nih.gov/pubmed/34062832
http://dx.doi.org/10.3390/ijms22094813
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author Dzimitrowicz, Anna
Jamroz, Piotr
Pohl, Pawel
Babinska, Weronika
Terefinko, Dominik
Sledz, Wojciech
Motyka-Pomagruk, Agata
author_facet Dzimitrowicz, Anna
Jamroz, Piotr
Pohl, Pawel
Babinska, Weronika
Terefinko, Dominik
Sledz, Wojciech
Motyka-Pomagruk, Agata
author_sort Dzimitrowicz, Anna
collection PubMed
description To the present day, no efficient plant protection method against economically important bacterial phytopathogens from the Pectobacteriaceae family has been implemented into agricultural practice. In this view, we have performed a multivariate optimization of the operating parameters of the reaction-discharge system, employing direct current atmospheric pressure glow discharge, generated in contact with a flowing liquid cathode (FLC-dc-APGD), for the production of a plasma-activated liquid (PAL) of defined physicochemical and anti-phytopathogenic properties. As a result, the effect of the operating parameters on the conductivity of PAL acquired under these conditions was assessed. The revealed optimal operating conditions, under which the PAL of the highest conductivity was obtained, were as follows: flow rate of the solution equaled 2.0 mL min(−1), the discharge current was 30 mA, and the inorganic salt concentration (ammonium nitrate, NH(4)NO(3)) in the solution turned out to be 0.50% (m/w). The developed PAL exhibited bacteriostatic and bactericidal properties toward Dickeya solani IFB0099 and Pectobacterium atrosepticum IFB5103 strains, with minimal inhibitory and minimal bactericidal concentrations equaling 25%. After 24 h exposure to 25% PAL, 100% (1−2 × 10(6)) of D. solani and P. atrosepticum cells lost viability. We attributed the antibacterial properties of PAL to the presence of deeply penetrating, reactive oxygen and nitrogen species (RONS), which were, in this case, OH, O, O(3), H(2)O(2), HO(2), NH, N(2), N(2)(+), NO(2)(−), NO(3)(−), and NH(4)(+). Putatively, the generated low-cost, eco-friendly, easy-to-store, and transport PAL, exhibiting the required antibacterial and physicochemical properties, may find numerous applications in the plant protection sector.
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spelling pubmed-81242192021-05-17 Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties Dzimitrowicz, Anna Jamroz, Piotr Pohl, Pawel Babinska, Weronika Terefinko, Dominik Sledz, Wojciech Motyka-Pomagruk, Agata Int J Mol Sci Article To the present day, no efficient plant protection method against economically important bacterial phytopathogens from the Pectobacteriaceae family has been implemented into agricultural practice. In this view, we have performed a multivariate optimization of the operating parameters of the reaction-discharge system, employing direct current atmospheric pressure glow discharge, generated in contact with a flowing liquid cathode (FLC-dc-APGD), for the production of a plasma-activated liquid (PAL) of defined physicochemical and anti-phytopathogenic properties. As a result, the effect of the operating parameters on the conductivity of PAL acquired under these conditions was assessed. The revealed optimal operating conditions, under which the PAL of the highest conductivity was obtained, were as follows: flow rate of the solution equaled 2.0 mL min(−1), the discharge current was 30 mA, and the inorganic salt concentration (ammonium nitrate, NH(4)NO(3)) in the solution turned out to be 0.50% (m/w). The developed PAL exhibited bacteriostatic and bactericidal properties toward Dickeya solani IFB0099 and Pectobacterium atrosepticum IFB5103 strains, with minimal inhibitory and minimal bactericidal concentrations equaling 25%. After 24 h exposure to 25% PAL, 100% (1−2 × 10(6)) of D. solani and P. atrosepticum cells lost viability. We attributed the antibacterial properties of PAL to the presence of deeply penetrating, reactive oxygen and nitrogen species (RONS), which were, in this case, OH, O, O(3), H(2)O(2), HO(2), NH, N(2), N(2)(+), NO(2)(−), NO(3)(−), and NH(4)(+). Putatively, the generated low-cost, eco-friendly, easy-to-store, and transport PAL, exhibiting the required antibacterial and physicochemical properties, may find numerous applications in the plant protection sector. MDPI 2021-05-01 /pmc/articles/PMC8124219/ /pubmed/34062832 http://dx.doi.org/10.3390/ijms22094813 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dzimitrowicz, Anna
Jamroz, Piotr
Pohl, Pawel
Babinska, Weronika
Terefinko, Dominik
Sledz, Wojciech
Motyka-Pomagruk, Agata
Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties
title Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties
title_full Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties
title_fullStr Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties
title_full_unstemmed Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties
title_short Multivariate Optimization of the FLC-dc-APGD-Based Reaction-Discharge System for Continuous Production of a Plasma-Activated Liquid of Defined Physicochemical and Anti-Phytopathogenic Properties
title_sort multivariate optimization of the flc-dc-apgd-based reaction-discharge system for continuous production of a plasma-activated liquid of defined physicochemical and anti-phytopathogenic properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124219/
https://www.ncbi.nlm.nih.gov/pubmed/34062832
http://dx.doi.org/10.3390/ijms22094813
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