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Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells

The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,0...

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Autores principales: Yamasaki, Masao, Yamasaki, Yumi, Furusho, Rina, Kimura, Hayaka, Kamei, Ichiro, Sonoda, Hiroko, Ikeda, Masahiro, Oshima, Tatsuya, Ogawa, Kenjiro, Nishiyama, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124543/
https://www.ncbi.nlm.nih.gov/pubmed/34067155
http://dx.doi.org/10.3390/molecules26092763
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author Yamasaki, Masao
Yamasaki, Yumi
Furusho, Rina
Kimura, Hayaka
Kamei, Ichiro
Sonoda, Hiroko
Ikeda, Masahiro
Oshima, Tatsuya
Ogawa, Kenjiro
Nishiyama, Kazuo
author_facet Yamasaki, Masao
Yamasaki, Yumi
Furusho, Rina
Kimura, Hayaka
Kamei, Ichiro
Sonoda, Hiroko
Ikeda, Masahiro
Oshima, Tatsuya
Ogawa, Kenjiro
Nishiyama, Kazuo
author_sort Yamasaki, Masao
collection PubMed
description The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,000× g and 200,000× g precipitates, and they contained quercetins, proteins, lipids, and small-sized RNA. The nanoparticles inhibited nitric oxide production from lipopolysaccharide (LPS)-stimulated RAW264 cells without cytotoxic properties. Cellular incorporation was confirmed by laser microscopic observation after PKH26 staining. The inhibition of caveolae-dependent endocytosis and macropinocytosis significantly prevented the incorporation of the nanoparticles but had no effect on the inhibition of nitric oxide in RAW264 cells. Collectively, the identified nanoparticles were capable of inhibiting the LPS response via extracellular mechanisms. Taken together, the way of consuming Allium cepa L. without collapsing the nanoparticles is expected to provide an efficient anti-inflammatory effect.
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spelling pubmed-81245432021-05-17 Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells Yamasaki, Masao Yamasaki, Yumi Furusho, Rina Kimura, Hayaka Kamei, Ichiro Sonoda, Hiroko Ikeda, Masahiro Oshima, Tatsuya Ogawa, Kenjiro Nishiyama, Kazuo Molecules Article The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,000× g and 200,000× g precipitates, and they contained quercetins, proteins, lipids, and small-sized RNA. The nanoparticles inhibited nitric oxide production from lipopolysaccharide (LPS)-stimulated RAW264 cells without cytotoxic properties. Cellular incorporation was confirmed by laser microscopic observation after PKH26 staining. The inhibition of caveolae-dependent endocytosis and macropinocytosis significantly prevented the incorporation of the nanoparticles but had no effect on the inhibition of nitric oxide in RAW264 cells. Collectively, the identified nanoparticles were capable of inhibiting the LPS response via extracellular mechanisms. Taken together, the way of consuming Allium cepa L. without collapsing the nanoparticles is expected to provide an efficient anti-inflammatory effect. MDPI 2021-05-07 /pmc/articles/PMC8124543/ /pubmed/34067155 http://dx.doi.org/10.3390/molecules26092763 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamasaki, Masao
Yamasaki, Yumi
Furusho, Rina
Kimura, Hayaka
Kamei, Ichiro
Sonoda, Hiroko
Ikeda, Masahiro
Oshima, Tatsuya
Ogawa, Kenjiro
Nishiyama, Kazuo
Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells
title Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells
title_full Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells
title_fullStr Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells
title_full_unstemmed Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells
title_short Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells
title_sort onion (allium cepa l.)-derived nanoparticles inhibited lps-induced nitrate production, however, their intracellular incorporation by endocytosis was not involved in this effect on raw264 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124543/
https://www.ncbi.nlm.nih.gov/pubmed/34067155
http://dx.doi.org/10.3390/molecules26092763
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