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The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
Cold preservation in University of Wisconsin (UW) solution is not enough to maintain the viability of the small intestine, due to the oxidative stress. The novel phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) has dual properties to reduce oxidative stress, radical scavenging, and...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124816/ https://www.ncbi.nlm.nih.gov/pubmed/34064340 http://dx.doi.org/10.3390/jcm10091972 |
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author | Fukai, Moto Nakayabu, Takuya Ohtani, Shintaro Shibata, Kengo Shimada, Shingo Sakamoto, Soudai Fuda, Hirotoshi Furukawa, Takayuki Watanabe, Mitsugu Hui, Shu-Ping Chiba, Hitoshi Shimamura, Tsuyoshi Taketomi, Akinobu |
author_facet | Fukai, Moto Nakayabu, Takuya Ohtani, Shintaro Shibata, Kengo Shimada, Shingo Sakamoto, Soudai Fuda, Hirotoshi Furukawa, Takayuki Watanabe, Mitsugu Hui, Shu-Ping Chiba, Hitoshi Shimamura, Tsuyoshi Taketomi, Akinobu |
author_sort | Fukai, Moto |
collection | PubMed |
description | Cold preservation in University of Wisconsin (UW) solution is not enough to maintain the viability of the small intestine, due to the oxidative stress. The novel phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) has dual properties to reduce oxidative stress, radical scavenging, and antioxidant protein induction, in other cells. This study was designed to determine whether DHMBA reduces cold preservation injury of enterocytes, and to identify the effector site. Enterocytes were subjected to 48-h cold preservation under atmosphere in UW solution (±DHMBA), and then returned to normal culture to replicate reperfusion of the small intestine after cold preservation. At the end of cold preservation (ECP) and at 1, 3, 6, and 72 h after rewarming (R1h, R3h, R6h, and R72h), we evaluated cell function and the injury mechanism. The results showed that DHMBA protected mitochondrial function mainly during cold preservation, and suppressed cell death after rewarming, as shown by the MTT, ATP, mitochondrial membrane potential, LDH, and lipid peroxidation assays, together with enhanced survival signals (PI3K, Akt, p70S6K) and induction of antioxidant proteins (HO-1, NQO-1, TRX-1). We found that DHMBA mitigates the cold-induced injury of enterocytes by protecting the mitochondria through direct and indirect antioxidative activities. |
format | Online Article Text |
id | pubmed-8124816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81248162021-05-17 The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions Fukai, Moto Nakayabu, Takuya Ohtani, Shintaro Shibata, Kengo Shimada, Shingo Sakamoto, Soudai Fuda, Hirotoshi Furukawa, Takayuki Watanabe, Mitsugu Hui, Shu-Ping Chiba, Hitoshi Shimamura, Tsuyoshi Taketomi, Akinobu J Clin Med Article Cold preservation in University of Wisconsin (UW) solution is not enough to maintain the viability of the small intestine, due to the oxidative stress. The novel phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) has dual properties to reduce oxidative stress, radical scavenging, and antioxidant protein induction, in other cells. This study was designed to determine whether DHMBA reduces cold preservation injury of enterocytes, and to identify the effector site. Enterocytes were subjected to 48-h cold preservation under atmosphere in UW solution (±DHMBA), and then returned to normal culture to replicate reperfusion of the small intestine after cold preservation. At the end of cold preservation (ECP) and at 1, 3, 6, and 72 h after rewarming (R1h, R3h, R6h, and R72h), we evaluated cell function and the injury mechanism. The results showed that DHMBA protected mitochondrial function mainly during cold preservation, and suppressed cell death after rewarming, as shown by the MTT, ATP, mitochondrial membrane potential, LDH, and lipid peroxidation assays, together with enhanced survival signals (PI3K, Akt, p70S6K) and induction of antioxidant proteins (HO-1, NQO-1, TRX-1). We found that DHMBA mitigates the cold-induced injury of enterocytes by protecting the mitochondria through direct and indirect antioxidative activities. MDPI 2021-05-04 /pmc/articles/PMC8124816/ /pubmed/34064340 http://dx.doi.org/10.3390/jcm10091972 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fukai, Moto Nakayabu, Takuya Ohtani, Shintaro Shibata, Kengo Shimada, Shingo Sakamoto, Soudai Fuda, Hirotoshi Furukawa, Takayuki Watanabe, Mitsugu Hui, Shu-Ping Chiba, Hitoshi Shimamura, Tsuyoshi Taketomi, Akinobu The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions |
title | The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions |
title_full | The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions |
title_fullStr | The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions |
title_full_unstemmed | The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions |
title_short | The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions |
title_sort | phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (dhmba) prevents enterocyte cell death under oxygen-dissolving cold conditions through polyphyletic antioxidant actions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124816/ https://www.ncbi.nlm.nih.gov/pubmed/34064340 http://dx.doi.org/10.3390/jcm10091972 |
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