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The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions

Cold preservation in University of Wisconsin (UW) solution is not enough to maintain the viability of the small intestine, due to the oxidative stress. The novel phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) has dual properties to reduce oxidative stress, radical scavenging, and...

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Autores principales: Fukai, Moto, Nakayabu, Takuya, Ohtani, Shintaro, Shibata, Kengo, Shimada, Shingo, Sakamoto, Soudai, Fuda, Hirotoshi, Furukawa, Takayuki, Watanabe, Mitsugu, Hui, Shu-Ping, Chiba, Hitoshi, Shimamura, Tsuyoshi, Taketomi, Akinobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124816/
https://www.ncbi.nlm.nih.gov/pubmed/34064340
http://dx.doi.org/10.3390/jcm10091972
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author Fukai, Moto
Nakayabu, Takuya
Ohtani, Shintaro
Shibata, Kengo
Shimada, Shingo
Sakamoto, Soudai
Fuda, Hirotoshi
Furukawa, Takayuki
Watanabe, Mitsugu
Hui, Shu-Ping
Chiba, Hitoshi
Shimamura, Tsuyoshi
Taketomi, Akinobu
author_facet Fukai, Moto
Nakayabu, Takuya
Ohtani, Shintaro
Shibata, Kengo
Shimada, Shingo
Sakamoto, Soudai
Fuda, Hirotoshi
Furukawa, Takayuki
Watanabe, Mitsugu
Hui, Shu-Ping
Chiba, Hitoshi
Shimamura, Tsuyoshi
Taketomi, Akinobu
author_sort Fukai, Moto
collection PubMed
description Cold preservation in University of Wisconsin (UW) solution is not enough to maintain the viability of the small intestine, due to the oxidative stress. The novel phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) has dual properties to reduce oxidative stress, radical scavenging, and antioxidant protein induction, in other cells. This study was designed to determine whether DHMBA reduces cold preservation injury of enterocytes, and to identify the effector site. Enterocytes were subjected to 48-h cold preservation under atmosphere in UW solution (±DHMBA), and then returned to normal culture to replicate reperfusion of the small intestine after cold preservation. At the end of cold preservation (ECP) and at 1, 3, 6, and 72 h after rewarming (R1h, R3h, R6h, and R72h), we evaluated cell function and the injury mechanism. The results showed that DHMBA protected mitochondrial function mainly during cold preservation, and suppressed cell death after rewarming, as shown by the MTT, ATP, mitochondrial membrane potential, LDH, and lipid peroxidation assays, together with enhanced survival signals (PI3K, Akt, p70S6K) and induction of antioxidant proteins (HO-1, NQO-1, TRX-1). We found that DHMBA mitigates the cold-induced injury of enterocytes by protecting the mitochondria through direct and indirect antioxidative activities.
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spelling pubmed-81248162021-05-17 The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions Fukai, Moto Nakayabu, Takuya Ohtani, Shintaro Shibata, Kengo Shimada, Shingo Sakamoto, Soudai Fuda, Hirotoshi Furukawa, Takayuki Watanabe, Mitsugu Hui, Shu-Ping Chiba, Hitoshi Shimamura, Tsuyoshi Taketomi, Akinobu J Clin Med Article Cold preservation in University of Wisconsin (UW) solution is not enough to maintain the viability of the small intestine, due to the oxidative stress. The novel phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) has dual properties to reduce oxidative stress, radical scavenging, and antioxidant protein induction, in other cells. This study was designed to determine whether DHMBA reduces cold preservation injury of enterocytes, and to identify the effector site. Enterocytes were subjected to 48-h cold preservation under atmosphere in UW solution (±DHMBA), and then returned to normal culture to replicate reperfusion of the small intestine after cold preservation. At the end of cold preservation (ECP) and at 1, 3, 6, and 72 h after rewarming (R1h, R3h, R6h, and R72h), we evaluated cell function and the injury mechanism. The results showed that DHMBA protected mitochondrial function mainly during cold preservation, and suppressed cell death after rewarming, as shown by the MTT, ATP, mitochondrial membrane potential, LDH, and lipid peroxidation assays, together with enhanced survival signals (PI3K, Akt, p70S6K) and induction of antioxidant proteins (HO-1, NQO-1, TRX-1). We found that DHMBA mitigates the cold-induced injury of enterocytes by protecting the mitochondria through direct and indirect antioxidative activities. MDPI 2021-05-04 /pmc/articles/PMC8124816/ /pubmed/34064340 http://dx.doi.org/10.3390/jcm10091972 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fukai, Moto
Nakayabu, Takuya
Ohtani, Shintaro
Shibata, Kengo
Shimada, Shingo
Sakamoto, Soudai
Fuda, Hirotoshi
Furukawa, Takayuki
Watanabe, Mitsugu
Hui, Shu-Ping
Chiba, Hitoshi
Shimamura, Tsuyoshi
Taketomi, Akinobu
The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
title The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
title_full The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
title_fullStr The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
title_full_unstemmed The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
title_short The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
title_sort phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (dhmba) prevents enterocyte cell death under oxygen-dissolving cold conditions through polyphyletic antioxidant actions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124816/
https://www.ncbi.nlm.nih.gov/pubmed/34064340
http://dx.doi.org/10.3390/jcm10091972
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