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Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat

This work studies the relationship between lung inflammation caused by nanomaterials and surfactant protein D (SP-D) kinetics and investigates whether SP-D can be a biomarker of the pulmonary toxicity of nanomaterials. Nanomaterials of nickel oxide and cerium dioxide were classified as having high t...

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Autores principales: Tomonaga, Taisuke, Izumi, Hiroto, Yoshiura, Yukiko, Nishida, Chinatsu, Yatera, Kazuhiro, Morimoto, Yasuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124995/
https://www.ncbi.nlm.nih.gov/pubmed/33924924
http://dx.doi.org/10.3390/ijms22094635
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author Tomonaga, Taisuke
Izumi, Hiroto
Yoshiura, Yukiko
Nishida, Chinatsu
Yatera, Kazuhiro
Morimoto, Yasuo
author_facet Tomonaga, Taisuke
Izumi, Hiroto
Yoshiura, Yukiko
Nishida, Chinatsu
Yatera, Kazuhiro
Morimoto, Yasuo
author_sort Tomonaga, Taisuke
collection PubMed
description This work studies the relationship between lung inflammation caused by nanomaterials and surfactant protein D (SP-D) kinetics and investigates whether SP-D can be a biomarker of the pulmonary toxicity of nanomaterials. Nanomaterials of nickel oxide and cerium dioxide were classified as having high toxicity, nanomaterials of two types of titanium dioxides and zinc oxide were classified as having low toxicity, and rat biological samples obtained from 3 days to 6 months after intratracheal instillation of those nanomaterials and micron-particles of crystalline silica were used. There were different tendencies of increase between the high- and low-toxicity materials in the concentration of SP-D in bronchoalveolar-lavage fluid (BALF) and serum and in the expression of the SP-D gene in the lung tissue. An analysis of the receiver operating characteristics for the toxicity of the nanomaterials by SP-D in BALF and serum showed a high accuracy of discrimination from 1 week to 3 or 6 months after exposure. These data suggest that the differences in the expression of SP-D in BALF and serum depended on the level of lung inflammation caused by the nanomaterials and that SP-D can be biomarkers for evaluating the pulmonary toxicity of nanomaterials.
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spelling pubmed-81249952021-05-17 Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat Tomonaga, Taisuke Izumi, Hiroto Yoshiura, Yukiko Nishida, Chinatsu Yatera, Kazuhiro Morimoto, Yasuo Int J Mol Sci Article This work studies the relationship between lung inflammation caused by nanomaterials and surfactant protein D (SP-D) kinetics and investigates whether SP-D can be a biomarker of the pulmonary toxicity of nanomaterials. Nanomaterials of nickel oxide and cerium dioxide were classified as having high toxicity, nanomaterials of two types of titanium dioxides and zinc oxide were classified as having low toxicity, and rat biological samples obtained from 3 days to 6 months after intratracheal instillation of those nanomaterials and micron-particles of crystalline silica were used. There were different tendencies of increase between the high- and low-toxicity materials in the concentration of SP-D in bronchoalveolar-lavage fluid (BALF) and serum and in the expression of the SP-D gene in the lung tissue. An analysis of the receiver operating characteristics for the toxicity of the nanomaterials by SP-D in BALF and serum showed a high accuracy of discrimination from 1 week to 3 or 6 months after exposure. These data suggest that the differences in the expression of SP-D in BALF and serum depended on the level of lung inflammation caused by the nanomaterials and that SP-D can be biomarkers for evaluating the pulmonary toxicity of nanomaterials. MDPI 2021-04-28 /pmc/articles/PMC8124995/ /pubmed/33924924 http://dx.doi.org/10.3390/ijms22094635 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tomonaga, Taisuke
Izumi, Hiroto
Yoshiura, Yukiko
Nishida, Chinatsu
Yatera, Kazuhiro
Morimoto, Yasuo
Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat
title Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat
title_full Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat
title_fullStr Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat
title_full_unstemmed Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat
title_short Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat
title_sort examination of surfactant protein d as a biomarker for evaluating pulmonary toxicity of nanomaterials in rat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124995/
https://www.ncbi.nlm.nih.gov/pubmed/33924924
http://dx.doi.org/10.3390/ijms22094635
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