Cargando…
Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin
A novel device for cholesteric liquid crystal (CLC)-based microfluidic chips, accommodated in a polydimethylsiloxane material, was invented. In this device, the reorientation of the CLCs was consistently influenced by the surface of the four channel walls and adjacent CLCs. When the inside of the mi...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125184/ https://www.ncbi.nlm.nih.gov/pubmed/34062757 http://dx.doi.org/10.3390/polym13091463 |
_version_ | 1783693424934780928 |
---|---|
author | Wang, I-Te Lee, Yen-Hua Chuang, Er-Yuan Hsiao, Yu-Cheng |
author_facet | Wang, I-Te Lee, Yen-Hua Chuang, Er-Yuan Hsiao, Yu-Cheng |
author_sort | Wang, I-Te |
collection | PubMed |
description | A novel device for cholesteric liquid crystal (CLC)-based microfluidic chips, accommodated in a polydimethylsiloxane material, was invented. In this device, the reorientation of the CLCs was consistently influenced by the surface of the four channel walls and adjacent CLCs. When the inside of the microchannel was coated with the alignment layer, the CLCs oriented homeotropically in a focal conic state under cross-polarizers. Once antigens had bound onto antibodies immobilized onto the orientation sheet-coated channel walls, the light intensity of the CLC molecules converted from a focal conic state to a bright planar state caused by disrupting the CLCs. By means of utilizing pressure-propelling flow, the attachment of antigen/antibody to the CLCs should be detectable within consecutive sequences. The multi-microfluidic CLC-based chips were verified by measuring bovine serum albumin (BSA) and immune complexes of pairs of BSA antigen/antibody. We showed that the multiple microfluidic immunoassaying can be used for measuring BSA and pairs of antigen/antibody BSA with a detection limit of about 1 ng/mL. The linear range is 0.1 μg/mL–1 mg/mL. A limit of immune detection of pairs of BSA antigens/antibodies was 10 ng/mL of BSA plus 1000 ng/mL of the anti-BSA antibodies was observed. According to this innovative creation of immunoassaying, an unsophisticated multi-detection device with CLC-based labeling-free microfluidic chips is presented. |
format | Online Article Text |
id | pubmed-8125184 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81251842021-05-17 Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin Wang, I-Te Lee, Yen-Hua Chuang, Er-Yuan Hsiao, Yu-Cheng Polymers (Basel) Article A novel device for cholesteric liquid crystal (CLC)-based microfluidic chips, accommodated in a polydimethylsiloxane material, was invented. In this device, the reorientation of the CLCs was consistently influenced by the surface of the four channel walls and adjacent CLCs. When the inside of the microchannel was coated with the alignment layer, the CLCs oriented homeotropically in a focal conic state under cross-polarizers. Once antigens had bound onto antibodies immobilized onto the orientation sheet-coated channel walls, the light intensity of the CLC molecules converted from a focal conic state to a bright planar state caused by disrupting the CLCs. By means of utilizing pressure-propelling flow, the attachment of antigen/antibody to the CLCs should be detectable within consecutive sequences. The multi-microfluidic CLC-based chips were verified by measuring bovine serum albumin (BSA) and immune complexes of pairs of BSA antigen/antibody. We showed that the multiple microfluidic immunoassaying can be used for measuring BSA and pairs of antigen/antibody BSA with a detection limit of about 1 ng/mL. The linear range is 0.1 μg/mL–1 mg/mL. A limit of immune detection of pairs of BSA antigens/antibodies was 10 ng/mL of BSA plus 1000 ng/mL of the anti-BSA antibodies was observed. According to this innovative creation of immunoassaying, an unsophisticated multi-detection device with CLC-based labeling-free microfluidic chips is presented. MDPI 2021-05-01 /pmc/articles/PMC8125184/ /pubmed/34062757 http://dx.doi.org/10.3390/polym13091463 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, I-Te Lee, Yen-Hua Chuang, Er-Yuan Hsiao, Yu-Cheng Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin |
title | Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin |
title_full | Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin |
title_fullStr | Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin |
title_full_unstemmed | Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin |
title_short | Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin |
title_sort | sensitive, color-indicating and labeling-free multi-detection cholesteric liquid crystal biosensing chips for detecting albumin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125184/ https://www.ncbi.nlm.nih.gov/pubmed/34062757 http://dx.doi.org/10.3390/polym13091463 |
work_keys_str_mv | AT wangite sensitivecolorindicatingandlabelingfreemultidetectioncholestericliquidcrystalbiosensingchipsfordetectingalbumin AT leeyenhua sensitivecolorindicatingandlabelingfreemultidetectioncholestericliquidcrystalbiosensingchipsfordetectingalbumin AT chuangeryuan sensitivecolorindicatingandlabelingfreemultidetectioncholestericliquidcrystalbiosensingchipsfordetectingalbumin AT hsiaoyucheng sensitivecolorindicatingandlabelingfreemultidetectioncholestericliquidcrystalbiosensingchipsfordetectingalbumin |